Iswara Raja Afw, Hestiantoro Andon, Budiningsih Yuli, Werdhani Retno A, Birowo Ponco, Wuyung Puspita E, Afandi Dedi
Doctoral Program in Medical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Department of Forensic and Medicolegal, Faculty of Medicine, Universitas Halu Oleo, Kendari, Indonesia.
Narra J. 2024 Aug;4(2):e823. doi: 10.52225/narra.v4i2.823. Epub 2024 Jun 21.
Sexual violence is a global issue affecting individuals regardless of their relationship to the perpetrator or the setting. Microscopic examination of spermatozoa from vaginal swabs is crucial in investigating cases of sexual intercourse to determine the time of the crime. Factors such as vaginal pH and neutrophil count influence the motility and persistence of spermatozoa in the vagina, particularly in conditions like candidiasis, highlighting the need for further research in this area. This study aimed to determine the effect of pH and neutrophil count on the motility and persistence of spermatozoa in the vagina with candidiasis. An experimental study was conducted using white rats () of the Wistar strain, with four male rats providing spermatozoa samples and 32 female rats receiving treatment. The female rats were divided into two groups: the normal group and the candidiasis model group. In both groups, the female rats were given vaginal insemination of spermatozoa. Variables measured included pH, neutrophil count, motility, and persistence of spermatozoa in the vagina. Data were analyzed using the Mann-Whitney test, followed by the Spearman correlation test. The findings revealed that spermatozoa motility lasted up to three minutes in normal rats, whereas in the candidiasis model, it was reduced to two minutes. Additionally, spermatozoa persistence in the vagina lasted up to six days in the normal group compared to up to three days in the candidiasis model. There were significant differences in pH, neutrophil count, motility, and persistence of spermatozoa in the vagina between the normal group and the candidiasis model (all had <0.001). There was a correlation between pH and neutrophil count with the motility and persistence of spermatozoa in the rat's vagina (<0.001). In conclusion, vaginal pH and neutrophil count influence the motility and persistence of spermatozoa in the vagina of candidiasis rat models.
性暴力是一个全球性问题，影响着个人，无论其与犯罪者的关系或所处环境如何。对阴道拭子中的精子进行显微镜检查对于调查性交案件以确定犯罪时间至关重要。阴道pH值和中性粒细胞计数等因素会影响精子在阴道内的活力和存活时间，尤其是在念珠菌病等情况下，这凸显了该领域进一步研究的必要性。本研究旨在确定pH值和中性粒细胞计数对念珠菌病大鼠阴道内精子活力和存活时间的影响。使用Wistar品系的白色大鼠进行了一项实验研究，4只雄性大鼠提供精子样本，32只雌性大鼠接受治疗。雌性大鼠分为两组：正常组和念珠菌病模型组。两组雌性大鼠均进行阴道内授精。测量的变量包括阴道内的pH值、中性粒细胞计数、精子活力和存活时间。数据采用Mann-Whitney检验进行分析，随后进行Spearman相关性检验。研究结果显示，正常大鼠的精子活力可持续3分钟，而在念珠菌病模型中则降至2分钟。此外，正常组阴道内精子的存活时间长达6天，而念珠菌病模型中则长达3天。正常组和念珠菌病模型组在阴道内的pH值、中性粒细胞计数、精子活力和存活时间方面存在显著差异（均<0.001）。大鼠阴道内的pH值和中性粒细胞计数与精子活力和存活时间之间存在相关性（<0.001）。总之，阴道pH值和中性粒细胞计数会影响念珠菌病大鼠模型阴道内精子的活力和存活时间。
