Ceyhan Veterinary Faculty, Department of Microbiology, Cukurova University, 01930, Adana, Turkey.
Present address: Microbiology Laboratory, Adana City Hospital, 01380, Adana, Turkey.
BMC Microbiol. 2018 Oct 24;18(1):155. doi: 10.1186/s12866-018-1305-6.
Rapid detection of sources and transmission routes by molecular methods provides key data for risk management of methicillin-resistant Staphylococcus aureus-induced infections acquired in both the community and hospitals. This study aimed to determine the clonal relationship of methicillin-resistant S. aureus strains isolated from our hospital by pulsed-field gel electrophoresis (PFGE) and Staphylococcal protein A (spa) typing methods and to identify the predominant clones in Cukurova Region, Turkey.
All isolates analyzed by PFGE were distributed among 11 clusters. Clusters A (n = 19) and B (n = 27) were 84.1% similar and accounted for 61% of all samples. All isolates were distributed among 18 spa types, with the most common type being t030 with 31 isolates (41.3%), followed by t223 with nine isolates (12%) and t127 with seven isolates (9.3%).
We found that t030 was the most common spa type in the area where the study was conducted, as also previously shown in studies undertaken in Turkey. However, the rate of t030 in our study was below the rates reported in the literature. We also detected some rare or sporadic spa types like t127, which has not been previously defined in our country. We consider that the spa typing and PFGE methods are useful for research on clonal relations in monitoring the changing prevalent clones in specific regions.
通过分子方法快速检测来源和传播途径，为社区和医院获得的耐甲氧西林金黄色葡萄球菌感染的风险管理提供关键数据。本研究旨在通过脉冲场凝胶电泳（PFGE）和葡萄球菌蛋白 A（spa）分型方法确定从我院分离的耐甲氧西林金黄色葡萄球菌菌株的克隆关系，并确定土耳其库鲁瓦拉地区的主要克隆。
通过 PFGE 分析的所有分离株分布在 11 个聚类中。聚类 A（n=19）和 B（n=27）相似性为 84.1%，占所有样本的 61%。所有分离株分布在 18 种 spa 型中，最常见的类型是 t030，有 31 株（41.3%），其次是 t223，有 9 株（12%）和 t127，有 7 株（9.3%）。
我们发现，t030 是研究地区最常见的 spa 型，在土耳其进行的研究中也有报道。然而，我们的研究中 t030 的比率低于文献报道的比率。我们还检测到一些罕见或散发性的 spa 型，如 t127，在我国以前没有定义过。我们认为 spa 分型和 PFGE 方法可用于研究监测特定地区流行克隆变化的克隆关系。
