Saito K, Yaginuma N, Takahashi T
Tohoku J Exp Med. 1979 Nov;129(3):273-83. doi: 10.1620/tjem.129.273.
The percentile volumes vA, vB and vD of A, B and D cells in the islets of Langerhans were histometrically estimated in the pancreases from 59 autopsy cases including maturity- and growth-onset diabetics and nondiabetics. Volumetry was performed microscopically by point counting method using a square-lattice eyepiece, then the total A, B and D cell volumes VA, VB, and VD were calculated from the total islet volume Vi of the pancreas in VB between the three groups. Namely, it was only 0.156 and 0.388 cm3 in the growth- and maturity-onset diabetics, much smaller values than 0.636 cm3 in nondiabetics. The simultaneous investigation on clinical records revealed a negative correlation between VB and the maximum blood sugar of the individual. The smaller VB in diabetes was attributed to the diminution of Vi because vA, vB and vD were almost the same regardless of the presence or absence of diabetes. These results strongly suggested a failure of insulin production in diabetes due to quantitative deficiency of B cells.
采用组织计量学方法，对59例尸检病例（包括成年发病型糖尿病患者、幼年发病型糖尿病患者和非糖尿病患者）胰腺中胰岛A、B和D细胞的百分体积vA、vB和vD进行了估计。使用方格目镜通过点计数法在显微镜下进行体积测定，然后根据三组胰腺中胰岛总体积Vi计算出A、B和D细胞的总体积VA、VB和VD。也就是说，幼年发病型糖尿病患者和成年发病型糖尿病患者的胰岛总体积分别仅为0.156和0.388 cm³，远低于非糖尿病患者的0.636 cm³。对临床记录的同步调查显示，VB与个体的最高血糖之间呈负相关。糖尿病患者的VB较小是由于Vi减小，因为无论是否患有糖尿病，vA、vB和vD几乎相同。这些结果有力地表明，糖尿病患者胰岛素分泌不足是由于B细胞数量缺乏。
