Acikgul Funda Cimen, Duran Nizami, Kutlu Tuncer, Ay Emrah, Tek Erhan, Bayraktar Suphi
Department of Medical Microbiology, Medical Faculty, Mustafa Kemal University, Antakya-Hatay, 31060, Turkey.
Department of Medical Microbiology, Medical Faculty, Agri İbrahim Cecen University, Agri 04100, Turkiye.
Heliyon. 2024 Aug 22;10(17):e36223. doi: 10.1016/j.heliyon.2024.e36223. eCollection 2024 Sep 15.
The purpose of this study is to investigate the potential anticarcinogenic effects of three phytochemicals, namely (AP), (GT), and (PC), on melanoma cells (A2058 cell line). Additionally, the study aims to explore the synergistic activities of these phytochemicals with Dacarbazine, a chemotherapy drug. To understand the molecular mechanism involved in apoptosis induction in the A-2058 cell line, it was used AO/EB staining for apoptosis detection and cell cycle analysis, monitored through flow cytometry. It also determined the mRNA expression levels of different apoptosis-regulatory genes, including , and caspase-3. The antitumor activities of these phytochemicals and their combinations were investigated in a subcutaneous mouse tumor model. The tumor diameter was 21.4 ± 1.1 mm in the Dacarbazine treatment group and 42.4 ± 3.1 mm in the control group. The antitumoral activities of AP and PC in the tumor model were similar to those of Dacarbazine. On the other hand, GT exhibited remarkable antitumoral activity, with a 1.75-fold reduction in tumor diameter compared to the Dacarbazine group. When different combinations of phytochemicals and Dacarbazine were used, the GT plus Dacarbazine treatment group was found to have a 3.5-fold reduction in tumor diameter compared to the Dacarbazine group. The tumor diameters in the Dacarbazine, AP plus GT, GT plus Dacarbazine, and AP plus Dacarbazine treatment groups were 21.4 ± 1.1, 7.6 ± 2.2, 8.6 ± 0.5, and 6.2 ± 1.9 mm, respectively.
本研究的目的是调查三种植物化学物质，即（此处原文中三种植物化学物质名称未给出完整，请补充完整后准确翻译）（AP）、（此处原文中植物化学物质名称未给出完整，请补充完整后准确翻译）（GT）和（此处原文中植物化学物质名称未给出完整，请补充完整后准确翻译）（PC）对黑色素瘤细胞（A2058细胞系）的潜在抗癌作用。此外，该研究旨在探索这些植物化学物质与化疗药物达卡巴嗪的协同活性。为了解A - 2058细胞系中凋亡诱导所涉及的分子机制，采用AO/EB染色进行凋亡检测和细胞周期分析，并通过流式细胞术进行监测。还测定了不同凋亡调节基因的mRNA表达水平，包括（此处原文中基因名称未给出完整，请补充完整后准确翻译）、（此处原文中基因名称未给出完整，请补充完整后准确翻译）和半胱天冬酶 - 3。在皮下小鼠肿瘤模型中研究了这些植物化学物质及其组合的抗肿瘤活性。达卡巴嗪治疗组的肿瘤直径为21.4±1.1毫米，对照组为42.4±3.1毫米。AP和PC在肿瘤模型中的抗肿瘤活性与达卡巴嗪相似。另一方面，GT表现出显著的抗肿瘤活性，与达卡巴嗪组相比，肿瘤直径减少了1.75倍。当使用植物化学物质和达卡巴嗪的不同组合时，发现GT加达卡巴嗪治疗组与达卡巴嗪组相比，肿瘤直径减少了3.5倍。达卡巴嗪、AP加GT、GT加Dacarbazine以及AP加Dacarbazine治疗组的肿瘤直径分别为21.4±1.1、7.6±2.2、8.6±0.5和6.2±1.9毫米。
