Wiraswati Hesti Lina, Ekawardhani Savira, Rohmawaty Enny, Laelalugina Amila, Zuhrotun Ade, Hendriani Rini, Wardhana Yoga Windhu, Bestari Muhammad Begawan, Sahirdjan Emmy Hermiyanti, Dewi Sumartini
Department of Biomedical Sciences, Universitas Padjadjaran, Sumedang, West Java, Indonesia.
Oncology and Stem Cell Working Group, Universitas Padjadjaran, Bandung, West Java, Indonesia.
J Inflamm Res. 2024 Sep 10;17:6297-6306. doi: 10.2147/JIR.S470318. eCollection 2024.
Linn. (Ciplukan) is a plant widely used in traditional medicine in subtropical and tropical regions. Most studies focus on its antioxidant and anti-inflammatory activity. Many studies also reported its therapeutic potential for treating cancer, malaria, hepatitis, rheumatism, liver problems, and tumors, but few studies have reported its anti-fibrosis activity. Here, we aimed to investigate the potential of as an antioxidant and anti-inflammatory that may be correlated with its anti-fibrosis action.
In our study, we treated 3T3-L1 and TGF-β-induced 3T3-L1 cells with an ethanol extract of . We then monitored the cell's response, evaluated the antioxidant activity using an MTT assay, and observed the cells' migration using the cell scratch assay. We used RT-PCR to determine the expression of HIF-1α and IL-6 on TGF-β-induced 3T3-L1 cells.
The ethanol extract of showed antioxidant activity and promoted cell proliferation on 3T3-L1 cells. Interestingly, the extract inhibited the migration of TGF-β-induced 3T3-L1 cells. Further analysis revealed that the extract could inhibit α expression and suppress expression on TGF-β-induced 3T3-L1 cells.
The ethanol extract of showed antioxidant and anti-inflammation activities in 3T3-L1 cells. Both activities are associated with the antifibrotic activity of ethanol extract.
Linn.（Ciplukan）是一种在亚热带和热带地区传统医学中广泛使用的植物。大多数研究集中在其抗氧化和抗炎活性上。许多研究还报道了其在治疗癌症、疟疾、肝炎、风湿病、肝脏问题和肿瘤方面的治疗潜力，但很少有研究报道其抗纤维化活性。在此，我们旨在研究其作为抗氧化剂和抗炎剂的潜力，这可能与其抗纤维化作用相关。
在我们的研究中，我们用的乙醇提取物处理3T3-L1细胞和转化生长因子-β（TGF-β）诱导的3T3-L1细胞。然后我们监测细胞的反应，使用MTT法评估抗氧化活性，并使用细胞划痕试验观察细胞的迁移。我们使用逆转录-聚合酶链反应（RT-PCR）来确定TGF-β诱导的3T3-L1细胞中缺氧诱导因子-1α（HIF-1α）和白细胞介素-6（IL-6）的表达。
的乙醇提取物显示出抗氧化活性，并促进了3T3-L1细胞的增殖。有趣的是，该提取物抑制了TGF-β诱导的3T3-L1细胞的迁移。进一步分析表明，该提取物可以抑制TGF-β诱导的3T3-L1细胞中α的表达并抑制的表达。
的乙醇提取物在3T3-L1细胞中显示出抗氧化和抗炎活性。这两种活性都与乙醇提取物的抗纤维化活性相关。
