Che Zain Mohamad Shazeli, Lee Soo Yee, Sarian Murni Nazira, Fakurazi Sharida, Shaari Khozirah
Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Laboratory of Vaccine and Immunotherapeutic, Institute of Bioscience, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Antioxidants (Basel). 2020 Apr 17;9(4):326. doi: 10.3390/antiox9040326.
Oil palm ( Jacq.) leaves (OPL) are widely available at zero cost in Southeast Asia countries, especially in Malaysia and Indonesia due to large scale oil palm plantations. OPLs contain a large amount of flavonoids in particular flavonoid -glycosides, which are known to possess useful biological properties including antioxidant and wound healing properties. The present study aimed at evaluating the wound healing efficacy of OPL in various solvent extracts and flavonoid enriched fractions and to determine the contribution of flavonoid -glycosides (orientin, isoorientin, vitexin and isovitexin) using scratch assay on 3T3 fibroblast cells. Solvent crude extracts with different polarity were screened and the most active extract was subjected to acid hydrolysis. The crude and acid hydrolysed extracts were further enriched using macroporous resins, XAD7HP. UHPLC-UV/PDA and LC-MS/MS analysis were applied for identification and confirmation of flavonoid -glycosides. The wound healing properties comprised of cell viability, cell proliferation and cell migration were studied. Allantoin was used as a positive control to compare the efficacy among the tested samples. The results revealed all OPL crude extracts, flavonoid enriched fractions and flavonoid -glycosides were non-toxic at concentrations below 25 µg/mL and showed better cell proliferation and migration activities at low concentrations than higher concentrations.. This study also demonstrated orientin, isoorientin, vitexin and isovitexin presented in OPL extracts and flavonoid enriched fractions stimulated proliferation and migration of 3T3 fibroblast cells. Hence, these findings may pose potential therapeutic bioactive agents for wound healing by enhancing fibroblast proliferation and migration.
由于大规模的油棕种植园，油棕（Jacq.）叶（OPL）在东南亚国家广泛可得且成本为零，尤其是在马来西亚和印度尼西亚。OPL含有大量的黄酮类化合物，特别是黄酮糖苷，已知其具有包括抗氧化和伤口愈合特性在内的有益生物学特性。本研究旨在评估OPL在各种溶剂提取物和富含黄酮类化合物的馏分中的伤口愈合功效，并使用划痕试验在3T3成纤维细胞上确定黄酮糖苷（荭草素、异荭草素、牡荆素和异牡荆素）的作用。筛选了具有不同极性的溶剂粗提物，并对活性最高的提取物进行酸水解。使用大孔树脂XAD7HP对粗提物和酸水解提取物进一步富集。应用超高效液相色谱-紫外/光电二极管阵列检测器（UHPLC-UV/PDA）和液相色谱-串联质谱（LC-MS/MS）分析来鉴定和确认黄酮糖苷。研究了包括细胞活力、细胞增殖和细胞迁移在内的伤口愈合特性。尿囊素用作阳性对照以比较测试样品之间的功效。结果显示，所有OPL粗提物、富含黄酮类化合物的馏分和黄酮糖苷在浓度低于25μg/mL时无毒，并且在低浓度下比高浓度下表现出更好的细胞增殖和迁移活性。本研究还表明，OPL提取物和富含黄酮类化合物的馏分中存在的荭草素、异荭草素、牡荆素和异牡荆素刺激了3T3成纤维细胞的增殖和迁移。因此，这些发现可能通过增强成纤维细胞的增殖和迁移为伤口愈合带来潜在的治疗性生物活性剂。
