Chairissy Marsha Dechastra, Wulandari Lely Retno, Sujuti Hidayat
Department of Ophthalmology, Faculty of Medicine, Brawijaya University, Malang 65145, Indonesia.
Int J Ophthalmol. 2019 Sep 18;12(9):1402-1407. doi: 10.18240/ijo.2019.09.05. eCollection 2019.
To investigate the effect of leaf extract on apoptotic and proliferation of retinoblastoma cells. Despite several previous studies evidencing the anti-cancer potential of ; however, certain study that proves its benefits in retinoblastoma cancer cells has been limited.
This study utilizes an experimental study by applying Y79 human retinoblastoma cell line culture obtained from the American Type Culture Collection (ATCC; 10801 University Boulevard Manassas, VA 20110, USA). The cell was divided into 4 groups. Group I was the control group without the administration of leaf extract. Whereas, group II, II and IV are engaged with 25, 50, and 100 µg/mL of leaf extract respectively. After a 24h incubation, an examination with microtetrazolium (MTT) cell proliferation assay and Annexin V apoptosis detection was conducted. Statistical analysis was performed with the Tukey test.
leaf extract improved apoptosis and significantly reduced the number of living cells in retinoblastoma cells, along with the increase in the given dose. Based on the Tukey test, a significant difference was found in the treatment group at 50 µg/mL (=0.025) and 100 µg/mL (=0.001) in the measurement of apoptosis. Proliferation measurements also indicated a significant decrease in the number of living cells in the 50µg/mL treatment group (=0.004), and in the 100 µg/mL treatment group (=0.000). Meanwhile, a dose of 25 µg/mL indicated insignificant difference in the two measurements. Improved apoptosis and decreased number of living cells occured at a dose of 100 µg/mL. Decreased number of living cells (in the measurement of proliferation) was due to the inhibited proliferation or improved apoptosis.
leaf extract improve apoptosis in retinoblastoma cell culture, requiring further research to inhibit proliferation.
探讨[植物名称]叶提取物对视网膜母细胞瘤细胞凋亡和增殖的影响。尽管此前有多项研究证明了[植物名称]的抗癌潜力；然而，证明其对视网膜母细胞瘤癌细胞有益的具体研究却很有限。
本研究采用实验研究方法，应用从美国典型培养物保藏中心（ATCC；美国弗吉尼亚州马纳萨斯大学大道10801号，邮编20110）获得的Y79人视网膜母细胞瘤细胞系进行培养。将细胞分为4组。第一组为对照组，不给予[植物名称]叶提取物。而第二组、第三组和第四组分别给予25、50和100μg/mL的[植物名称]叶提取物。孵育24小时后，进行微量四氮唑蓝（MTT）细胞增殖测定和膜联蛋白V凋亡检测。采用Tukey检验进行统计分析。
[植物名称]叶提取物可改善视网膜母细胞瘤细胞的凋亡，并随着给药剂量的增加显著减少活细胞数量。根据Tukey检验，在凋亡测定中，50μg/mL（P = 0.025）和100μg/mL（P = 0.001）的治疗组存在显著差异。增殖测定也表明，50μg/mL治疗组（P = 0.004）和100μg/mL治疗组（P = 0.000）的活细胞数量显著减少。同时，25μg/mL剂量在两项测定中均无显著差异。在100μg/mL剂量下出现凋亡改善和活细胞数量减少。活细胞数量减少（在增殖测定中）是由于增殖受到抑制或凋亡得到改善。
[植物名称]叶提取物可改善视网膜母细胞瘤细胞培养中的凋亡，抑制增殖还需进一步研究。
