Oshamika Oyewumi, Sonowo Oreoluwa, Akinbolagbe Yeside, Odusote Olatunde, Akinnola Olayemi, Eni Angela
Department of Biological Sciences, College of Science and Technology, Covenant University, Canaan Land, KM 10 Idiroko Rd, Ota, 112104 Ogun State Nigeria.
Department of Paediatrics, Lagos University Teaching Hospital, Idi-Araba, Lagos State Nigeria.
Indian J Microbiol. 2024 Sep;64(3):1144-1152. doi: 10.1007/s12088-024-01272-z. Epub 2024 Apr 18.
Asthma is a chronic respiratory disease that affects children worldwide. Increasing evidence suggests that contributes to the pathology of asthma. The aim of this study was to evaluate the nasal carriage, antimicrobial susceptibility profile, and presence of enterotoxin genes from isolated from children with asthma. Nasal swab samples were collected from 158 children, including 98 children with asthma and 60 healthy controls. isolates were identified using phenotypic methods and the presence of the gene. Antimicrobial susceptibility testing was performed using the Kirby-Bauer disc diffusion method. Polymerase chain reaction (PCR) confirmed the presence of the A gene and enterotoxin genes. The gene was confirmed in 83 isolates, resulting in a nasal carriage of 52.5% (83/158). The nasal carriage of was higher among asthma cases (72.4%), with a significant association of nasal carriage observed among asthma cases (OR 0.201, 95% CI 0.063-0.645, = 0.007). Methicillin-resistant (MRSA) nasal carriage was 11.4%. The isolates showed high resistance to cefoxitin (99%) and penicillin (92%) but were sensitive to gentamicin (25%). Furthermore, 67.5% of the isolates were multi-drug resistant. The staphylococcal enterotoxin c gene () was the most prevalent enterotoxin (19.7%) among cases and controls. These findings highlight the need for improved antibiotic stewardship in paediatric medicine and implementation of infection control policies.
The online version contains supplementary material available at 10.1007/s12088-024-01272-z.
哮喘是一种影响全球儿童的慢性呼吸道疾病。越来越多的证据表明，[具体因素未明确写出]导致哮喘的病理过程。本研究的目的是评估从哮喘儿童中分离出的[细菌名称未明确写出]的鼻腔携带情况、抗菌药物敏感性概况以及肠毒素基因的存在情况。从158名儿童中采集鼻拭子样本，其中包括98名哮喘儿童和60名健康对照。使用表型方法和[基因名称未明确写出]基因鉴定[细菌名称未明确写出]分离株。采用 Kirby-Bauer 纸片扩散法进行抗菌药物敏感性测试。聚合酶链反应（PCR）证实了 A 基因和肠毒素基因的存在。在83株分离株中证实了[基因名称未明确写出]基因，鼻腔携带率为52.5%（83/158）。哮喘病例中[细菌名称未明确写出]的鼻腔携带率更高（72.4%），在哮喘病例中观察到[细菌名称未明确写出]鼻腔携带存在显著关联（比值比0.201，95%置信区间0.063 - 0.645，P = 0.007）。耐甲氧西林[细菌名称未明确写出]（MRSA）鼻腔携带率为11.4%。[细菌名称未明确写出]分离株对头孢西丁（99%）和青霉素（92%）表现出高度耐药，但对庆大霉素敏感（25%）。此外，67.5%的分离株具有多重耐药性。葡萄球菌肠毒素 c 基因（[基因名称未明确写出]）是病例和对照中最常见的肠毒素（19.7%）。这些发现凸显了改善儿科医学中抗生素管理以及实施感染控制政策的必要性。
在线版本包含可在10.1007/s12088 - 024 - 01272 - z获取的补充材料。
