西高止山脉森林土壤中需氧趋磁细菌的筛选、分离与鉴定
Screening, Isolation and Characterization of Aerobic Magnetotactic Bacteria From Western Ghats Forest Soil.
作者信息
Mistry Kruti, Markande Anoop R, Patel Janki K, Parekh Kinnari
机构信息
Department of Biological Sciences, P.D. Patel Institute of Applied Sciences (PDPIAS), Charotar University of Science and Technology (CHARUSAT), Changa, Gujarat 388421 India.
Present Address: Department of Microbiology, School of Basic & Applied Sciences, B- Block, Innovation Campus, Dayananda Sagar University, Kudlu Gate, Hosur Road, Bengaluru, 560068 India.
出版信息
Indian J Microbiol. 2024 Sep;64(3):1257-1265. doi: 10.1007/s12088-024-01316-4. Epub 2024 May 27.
UNLABELLED
Magnetotactic bacteria (MTB) are a unique ecophysiological group of iron-metabolizing bacteria that have immense potential biotechnological applications. These bacteria have predominantly been isolated from oxygen-limited conditions of aquatic niches and rarely from the soils. The Western Ghats biodiversity hotspot has been well-studied for its fauna and flora diversity. The present study includes optimization of enrichment medium for cultivation of MTB, to suit aerobic mesophiles from forest soil. The major components included were Ferric quinate and Resazurin. The enrichment and isolates were characterized for their magnetic properties using magnetotaxis on agar plates, Vibrating Sampler Magnetometer (VSM), and X-ray diffraction (XRD) analysis. The isolates, namely sp. S1 (MN212953), sp. S2_03 (MN212954), sp. S2_08 (MN212955) and sp. S2_09 (MN212956) were isolated and characterized to have a magnetosome size of 2.5-8 nm. Our study is the first report on the enrichment and isolation of MTB from Western Ghats forest soil.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s12088-024-01316-4.
未标记
趋磁细菌（MTB）是一类独特的铁代谢细菌生态生理群体，具有巨大的生物技术应用潜力。这些细菌主要从水生生态位的缺氧环境中分离出来，很少从土壤中分离得到。西高止山脉生物多样性热点地区的动植物多样性已得到充分研究。本研究包括优化用于培养趋磁细菌的富集培养基，以适应森林土壤中的需氧嗜温菌。主要成分包括奎宁酸铁和刃天青。利用琼脂平板上的趋磁性、振动采样磁力计（VSM）和X射线衍射（XRD）分析对富集物和分离物的磁性进行了表征。分离得到的菌株分别为sp. S1（MN212953）、sp. S2_03（MN212954）、sp. S2_08（MN212955）和sp. S2_09（MN212956），经表征其磁小体大小为2.5 - 8纳米。我们的研究是关于从西高止山脉森林土壤中富集和分离趋磁细菌的首次报道。
补充信息
在线版本包含可在10.1007/s12088 - 024 - 01316 - 4获取的补充材料。
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