Volk H D, Waschke S R, Diezel W, Grunow R, von Baehr R, Fiebig H
Immunol Lett. 1985;10(2):103-7. doi: 10.1016/0165-2478(85)90184-1.
The kinetics of HLA-DR antigen expression by human peripheral blood monocytes during cultivation in vitro was studied. Immediately after separation by glass adherence, about 60% of monocytes expressed DR antigens as judged by indirect fluorescence staining with a monoclonal antibody (BL-DR/1). Monocytes carefully depleted of lymphocytes, gradually lost their DR antigens during cultivation in the absence of exogenous interferon-gamma (IFN-gamma). However, addition of a few lymphocytes to the adherent cells prevented the decrease of DR antigen expression. Furthermore, it was shown that doses as low as 1 IU/ml of IFN-gamma are sufficient to induce DR antigen expression by cultured monocytes. Experiments with cyclosporin A suggest that lymphocytes contaminating the monocyte preparations can produce spontaneously sufficient amounts of IFN-gamma for maintenance of the DR antigens on monocytes.
研究了人外周血单核细胞在体外培养期间HLA - DR抗原表达的动力学。通过玻璃黏附分离后，立即用单克隆抗体（BL - DR/1）进行间接荧光染色判断，约60%的单核细胞表达DR抗原。小心去除淋巴细胞的单核细胞，在无外源性干扰素 - γ（IFN - γ）的培养过程中逐渐失去其DR抗原。然而，向贴壁细胞中加入少量淋巴细胞可防止DR抗原表达的降低。此外，研究表明，低至1 IU/ml的IFN - γ剂量就足以诱导培养的单核细胞表达DR抗原。用环孢素A进行的实验表明，污染单核细胞制剂的淋巴细胞可自发产生足够量的IFN - γ以维持单核细胞上的DR抗原。
