Hutter K J, Stoehr M
Histochemistry. 1985;82(5):469-75. doi: 10.1007/BF02450482.
The chromosomes of WALKER (W-256) carcinoma cells have been separated into different DNA subclasses using DAPI for quantitative DNA staining and laser flow cytometry. The submetacentric marker chromosome could be isolated and its DNA content was determined to be 1.3 pg. One microgram marker DNA was obtained after separation of about 750 000 marker chromosomes by means of electronic flow sorting. The chromosomal composition of sorted fractions was analyzed by microscopy following banding of sorted chromosomes. The average morphological purity obtained was about 83%.
利用4',6-二脒基-2-苯基吲哚（DAPI）进行DNA定量染色，并结合激光流式细胞术，已将沃克（W-256）癌细胞的染色体分离为不同的DNA亚类。近中着丝粒标记染色体能够被分离出来，其DNA含量测定为1.3皮克。通过电子流分选大约750,000条标记染色体后，获得了1微克标记DNA。对分选后的染色体进行显带处理后，通过显微镜分析了分选组分的染色体组成。所获得的平均形态纯度约为83%。
