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剪接因子SRs相关基因特征的综合分析：预测骨肉瘤预后及免疫调节状态

Comprehensive analysis of splicing factor SRs-related gene characteristics: predicting osteosarcoma prognosis and immune regulation status.

作者信息

Long Changhai, Ma Biao, Li Kai, Liu Sijing

机构信息

Department of Orthopedic Center, The Second Hospital Affiliated to Guangdong Medical University, Zhanjiang, Guangdong, China.

出版信息

Front Oncol. 2024 Sep 2;14:1456986. doi: 10.3389/fonc.2024.1456986. eCollection 2024.

DOI:10.3389/fonc.2024.1456986
PMID:39286028
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11403285/
Abstract

OBJECTIVE

To investigate the impact of SRs-related genes on the overall survival and prognosis of osteosarcoma patients through bulk and single-cell RNA-seq transcriptome analysis.

METHODS

In this study, we constructed a prognosis model based on serine/arginine-rich splicing factors (SRs) and predicted the survival of osteosarcoma patients. By analyzing single-cell RNA sequencing data and applying AUCell enrichment analysis, we revealed oncogenic pathways of SRs in osteosarcoma immune cells. Additionally, we described the regulatory role of SRSF7 in pan-cancer.

RESULTS

Lasso regression analysis identified 6 key SRs-related genes, and a prognosis prediction model was established. The upregulation of these pathways revealed that SRs promote tumor cell proliferation and survival by regulating related signaling pathways and help tumor cells evade host immune surveillance. Additionally, by grouping single-cell data using AUCell, we found significant differences in T cell expression between high and low-risk groups. The analysis results indicated that the regulatory activity of SRs is closely related to T cell function, particularly in regulating immune responses and promoting immune evasion. Furthermore, SRSF7 regulates cell proliferation and apoptosis.

CONCLUSION

SRs-related genes play a critical regulatory role in osteosarcoma. T cells are key in regulating immune responses and promoting immune evasion through SRs genes. SRSF7 is a significant gene influencing the occurrence and development of osteosarcoma.

摘要

目的

通过批量和单细胞RNA测序转录组分析，研究富含丝氨酸/精氨酸的剪接因子（SRs）相关基因对骨肉瘤患者总生存期和预后的影响。

方法

在本研究中，我们构建了基于丝氨酸/精氨酸丰富剪接因子（SRs）的预后模型，并预测骨肉瘤患者的生存情况。通过分析单细胞RNA测序数据并应用AUCell富集分析，我们揭示了SRs在骨肉瘤免疫细胞中的致癌途径。此外，我们描述了SRSF7在泛癌中的调控作用。

结果

套索回归分析确定了6个关键的SRs相关基因，并建立了预后预测模型。这些途径的上调表明，SRs通过调节相关信号通路促进肿瘤细胞增殖和存活，并帮助肿瘤细胞逃避宿主免疫监视。此外，通过使用AUCell对单细胞数据进行分组，我们发现高风险组和低风险组之间T细胞表达存在显著差异。分析结果表明，SRs的调控活性与T细胞功能密切相关，特别是在调节免疫反应和促进免疫逃逸方面。此外，SRSF7调节细胞增殖和凋亡。

结论

SRs相关基因在骨肉瘤中起关键调控作用。T细胞是通过SRs基因调节免疫反应和促进免疫逃逸的关键因素。SRSF7是影响骨肉瘤发生发展的重要基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6db/11403285/c8bc6cc1da16/fonc-14-1456986-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6db/11403285/c8bc6cc1da16/fonc-14-1456986-g008.jpg
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Molecular characteristics and clinical implications of serine/arginine-rich splicing factors in human cancer.
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