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基于生物聚合物的纳米颗粒提高了蒲桃(Syzygium cumini (L.) Skeels)中酚类化合物的抗氧化活性。

Nanoparticles based on biopolymers improved antioxidant activity of phenolic compounds from jambolan ( (L.) skeels).

作者信息

Barbosa Dos Santos Jessica Anarellis, Assis Cristiane Fernandes, Soares Aragao Cicero Flavio, Dos Santos Lima Marcos, Passos Thais Souza, da Silva-Maia Juliana Kelly

机构信息

Postgraduate Program in Nutrition, Center for Health Sciences, Federal University of Rio Grande do Norte (UFRN), 59078-900, Natal, Brazil.

Department of Nutrition, Center for Health Sciences, Federal University of Rio Grande do Norte (UFRN), 59078-900, Natal, Brazil.

出版信息

Heliyon. 2024 Aug 28;10(17):e36973. doi: 10.1016/j.heliyon.2024.e36973. eCollection 2024 Sep 15.

Abstract

Jambolan ( L.) is an underutilized fruit rich in bioactive phenolic compounds, specially anthocyanins, but the low stability of these substances and interaction with other compounds in the food matrix limit their application as food additives; nanoencapsulation is the best strategy to overcome these limitations. This study aimed to nanoencapsulate a phenolic-rich jambolan extract using whey proteins and pectin by nanoprecipitation in different antisolvent compositions. Two formulations were synthesized (7.33 % extract, 1.67 % pectin, and 5 % concentrated or isolated whey protein) precipitated in different acetone concentrations (50, 70, and 100 % v/v). SEM showed particles with spherical shape and smooth surface. DLS pointed diameters between 82 nm and 116 nm. FTIR indicated chemical interactions between the materials. Encapsulation efficiency showed high phenolic compounds entrapment in all systems [73.81-84.65 %, p > 0.05]. However, particles precipitated in 50 and 100 % acetone (v/v) showed greater anthocyanins retention [56.89-35.24 %, p < 0.05]. Nanoencapsulation potentiated the antioxidant activity up to 110 % more than the crude extract (p < 0.05). These results show the potential of nanoprecipitation as an effective encapsulation process and the biopolymers combination to produce nanoparticles containing jambolan phenolic compounds to promote their application in foods and health products.

摘要

蒲桃(Syzygium cumini (L.) Skeels)是一种未得到充分利用的水果,富含生物活性酚类化合物,特别是花青素,但这些物质稳定性低且与食品基质中的其他化合物相互作用,限制了它们作为食品添加剂的应用;纳米包封是克服这些限制的最佳策略。本研究旨在通过在不同反溶剂组成中进行纳米沉淀,使用乳清蛋白和果胶对富含酚类的蒲桃提取物进行纳米包封。合成了两种配方(7.33%提取物、1.67%果胶和5%浓缩或分离乳清蛋白),在不同丙酮浓度(50、70和100% v/v)下沉淀。扫描电子显微镜(SEM)显示颗粒呈球形且表面光滑。动态光散射(DLS)表明粒径在82纳米至116纳米之间。傅里叶变换红外光谱(FTIR)表明材料之间存在化学相互作用。包封效率显示所有体系中酚类化合物的包封率都很高[73.81 - 84.65%,p > 0.05]。然而,在50%和100%丙酮(v/v)中沉淀的颗粒显示出更高的花青素保留率[56.89 - 35.24%,p < 0.05]。纳米包封使抗氧化活性比粗提物提高了多达110%(p < 0.05)。这些结果表明纳米沉淀作为一种有效的包封工艺以及生物聚合物组合用于生产含有蒲桃酚类化合物的纳米颗粒以促进其在食品和保健品中的应用具有潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3a7/11402765/43a7168b587e/ga1.jpg

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