• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

复杂鞘脂谱分析及一种肌醇磷酸神经酰胺合酶的鉴定。 （原文中“in.”后面内容缺失，翻译根据现有内容尽量完整呈现）

Complex sphingolipid profiling and identification of an inositol-phosphorylceramide synthase in .

作者信息

Listian Stevanus A, Mazur Anna-Carina, Kol Matthijs, Ufelmann Edwin, Eising Sebastian, Fröhlich Florian, Walter Stefan, Holthuis Joost C M, Barisch Caroline

机构信息

Division of Molecular Infection Biology, Department of Biology/Chemistry, University of Osnabrück, Osnabrück, Germany.

Centre for Structural Systems Biology, Hamburg, Germany.

出版信息

iScience. 2024 Jul 30;27(9):110609. doi: 10.1016/j.isci.2024.110609. eCollection 2024 Sep 20.

DOI:10.1016/j.isci.2024.110609
PMID:39286488
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11402645/
Abstract

is a professional phagocyte frequently used to study cellular processes underlying the recognition, engulfment, and infection course of microbial pathogens. Sphingolipids are abundant components of the plasma membrane that bind cholesterol, control membrane properties, participate in signal transmission, and serve as adhesion molecules in recognition processes relevant to immunity and infection. By combining lipidomics with a bioinformatics-based cloning strategy, we show here that produces phosphoinositol-containing sphingolipids with predominantly phytoceramide backbones. Cell-free expression of candidate inositol-phosphorylceramide (IPC) synthases from enabled identification of an enzyme that selectively catalyzes the transfer of phosphoinositol from phosphatidylinositol onto ceramide. The IPC synthase, IPCS1, shares multiple sequence motifs with yeast IPC and human sphingomyelin synthases and localizes to the Golgi apparatus as well as the contractile vacuole of . These findings open up important opportunities for exploring a role of sphingolipids in phagocytosis and infection across major evolutionary boundaries.

摘要

是一种专业吞噬细胞，常用于研究微生物病原体识别、吞噬和感染过程背后的细胞过程。鞘脂是质膜的丰富成分，可结合胆固醇、控制膜特性、参与信号传递，并在与免疫和感染相关的识别过程中充当粘附分子。通过将脂质组学与基于生物信息学的克隆策略相结合，我们在此表明，产生主要以植物神经酰胺为骨架的含磷酸肌醇鞘脂。来自的候选肌醇磷酸神经酰胺（IPC）合酶的无细胞表达能够鉴定出一种酶，该酶选择性地催化磷酸肌醇从磷脂酰肌醇转移到神经酰胺上。IPC合酶IPCS1与酵母IPC和人鞘磷脂合酶具有多个序列基序，并定位于的高尔基体以及收缩泡。这些发现为探索鞘脂在跨越主要进化界限的吞噬作用和感染中的作用提供了重要机会。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/be19e116b6fb/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/0896ab1cb993/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/3a9d5183224c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/dd8077a30b58/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/8fde52f96119/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/3805b1311245/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/bf042ab94646/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/1ddf04ff4f16/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/2bcfda763efe/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/be19e116b6fb/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/0896ab1cb993/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/3a9d5183224c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/dd8077a30b58/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/8fde52f96119/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/3805b1311245/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/bf042ab94646/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/1ddf04ff4f16/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/2bcfda763efe/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c39/11402645/be19e116b6fb/gr8.jpg

相似文献

1
Complex sphingolipid profiling and identification of an inositol-phosphorylceramide synthase in .复杂鞘脂谱分析及一种肌醇磷酸神经酰胺合酶的鉴定。 （原文中“in.”后面内容缺失，翻译根据现有内容尽量完整呈现）
iScience. 2024 Jul 30;27(9):110609. doi: 10.1016/j.isci.2024.110609. eCollection 2024 Sep 20.
2
Sphingolipid synthesis as a target for antifungal drugs. Complementation of the inositol phosphorylceramide synthase defect in a mutant strain of Saccharomyces cerevisiae by the AUR1 gene.鞘脂合成作为抗真菌药物的靶点。AUR1基因对酿酒酵母突变株中肌醇磷酸神经酰胺合酶缺陷的互补作用。
J Biol Chem. 1997 Apr 11;272(15):9809-17. doi: 10.1074/jbc.272.15.9809.
3
The protozoan inositol phosphorylceramide synthase: a novel drug target that defines a new class of sphingolipid synthase.原生动物肌醇磷酸神经酰胺合酶：一种新型药物靶点，定义了一类新的鞘脂合酶。
J Biol Chem. 2006 Sep 22;281(38):28200-9. doi: 10.1074/jbc.M600796200. Epub 2006 Jul 22.
4
Protein sorting in the late Golgi of Saccharomyces cerevisiae does not require mannosylated sphingolipids.酿酒酵母晚期高尔基体中的蛋白质分选不需要甘露糖基化鞘脂。
J Biol Chem. 2004 Jan 9;279(2):1020-9. doi: 10.1074/jbc.M306119200. Epub 2003 Oct 28.
5
An overview of sphingolipid metabolism: from synthesis to breakdown.鞘脂代谢概述：从合成到分解。
Adv Exp Med Biol. 2010;688:1-23. doi: 10.1007/978-1-4419-6741-1_1.
6
Cell-free synthesis and functional characterization of sphingolipid synthases from parasitic trypanosomatid protozoa.无细胞合成及寄生原生动物锥虫鞘脂合酶的功能特征分析。
J Biol Chem. 2010 Jul 2;285(27):20580-7. doi: 10.1074/jbc.M110.127662. Epub 2010 May 10.
7
Developmentally regulated sphingolipid synthesis in African trypanosomes.非洲锥虫中发育调控的鞘脂合成
Mol Microbiol. 2008 Oct;70(2):281-96. doi: 10.1111/j.1365-2958.2008.06393.x. Epub 2008 Aug 11.
8
Sphingolipid synthesis and scavenging in the intracellular apicomplexan parasite, Toxoplasma gondii.细胞内顶复门寄生虫刚地弓形虫中的鞘脂合成与清除
Mol Biochem Parasitol. 2013 Jan;187(1):43-51. doi: 10.1016/j.molbiopara.2012.11.007. Epub 2012 Dec 16.
9
Inositol phosphorylceramide synthase null Leishmania are viable and virulent in animal infections where salvage of host sphingomyelin predominates.肌醇磷酸神经酰胺合酶缺失的利什曼原虫在动物感染中是可行和有毒的，在这些感染中，宿主神经鞘磷脂的挽救占主导地位。
J Biol Chem. 2022 Nov;298(11):102522. doi: 10.1016/j.jbc.2022.102522. Epub 2022 Sep 23.
10
Mannosylinositol phosphorylceramide is a major sphingolipid component and is required for proper localization of plasma-membrane proteins in Schizosaccharomyces pombe.甘露糖基肌醇磷酸神经酰胺是一种主要的鞘脂类成分，是裂殖酵母（Schizosaccharomyces pombe）质膜蛋白正确定位所必需的。
J Cell Sci. 2010 May 1;123(Pt 9):1578-87. doi: 10.1242/jcs.059139. Epub 2010 Apr 13.

本文引用的文献

1
Structure of the ceramide-bound SPOTS complex.神经酰胺结合的SPOTS复合物的结构。
Nat Commun. 2023 Oct 4;14(1):6196. doi: 10.1038/s41467-023-41747-z.
2
Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathway.鞘磷脂合成酶 SMS2 的致病性变异破坏分泌途径中的脂质景观。
Elife. 2022 Sep 14;11:e79278. doi: 10.7554/eLife.79278.
3
Mutant resources for functional genomics in Dictyostelium discoideum using REMI-seq technology.利用 REMI-seq 技术在盘基网柄菌中进行功能基因组学的突变资源研究。
BMC Biol. 2021 Aug 24;19(1):172. doi: 10.1186/s12915-021-01108-y.
4
Highly accurate protein structure prediction with AlphaFold.利用 AlphaFold 进行高精度蛋白质结构预测。
Nature. 2021 Aug;596(7873):583-589. doi: 10.1038/s41586-021-03819-2. Epub 2021 Jul 15.
5
Targeted Lipidomics of Drosophila melanogaster During Development.黑腹果蝇发育过程中的靶向脂质组学
Methods Mol Biol. 2021;2306:187-213. doi: 10.1007/978-1-0716-1410-5_13.
6
Sphingolipids in plants: a guidebook on their function in membrane architecture, cellular processes, and environmental or developmental responses.植物中的鞘脂类：关于它们在膜结构、细胞过程以及环境或发育响应中的功能的指南。
FEBS Lett. 2020 Nov;594(22):3719-3738. doi: 10.1002/1873-3468.13987. Epub 2020 Nov 24.
7
Phospholipids containing ether-bound hydrocarbon-chains are essential for efficient phagocytosis and neutral lipids of the ester-type perturb development in .含醚键连接烃链的磷脂对于有效的吞噬作用是必不可少的，而酯型的中性脂会干扰 的发育。
Biol Open. 2020 Jul 16;9(7):bio052126. doi: 10.1242/bio.052126.
8
LipidCreator workbench to probe the lipidomic landscape.利用 LipidCreator 工作台探索脂质组学全景。
Nat Commun. 2020 Apr 28;11(1):2057. doi: 10.1038/s41467-020-15960-z.
9
A Stroll Down the CerS Lane.沿着 CerS 通路漫步。
Adv Exp Med Biol. 2019;1159:49-63. doi: 10.1007/978-3-030-21162-2_4.
10
On the road to unraveling the molecular functions of ether lipids.在揭示醚脂分子功能的道路上。
FEBS Lett. 2019 Sep;593(17):2378-2389. doi: 10.1002/1873-3468.13465. Epub 2019 Jun 13.