Dardenne Eye Clinic, Bonn, Germany.
Deutsches Rheumaforschungszentrum, DRFZ, Berlin, Germany.
Invest Ophthalmol Vis Sci. 2024 Sep 3;65(11):25. doi: 10.1167/iovs.65.11.25.
To investigate if the cytokine profile in the aqueous humor (AH) of cataract patients varies according to cataract type and severity.
This prospective study included 397 eyes of 397 patients (median age, 76 years; range, 30-94 years) who underwent standard small-incision phacoemulsification surgery. Cataracts were graded using the LOCS III system: mild (≤3), moderate (3.5-5), and severe (≥5). Biometric measurements from the IOL master 700 (Zeiss, Oberkochen, Germany) were used to differentiate between thick (>4.5 mm) and thin lenses. Information about age, gender, and self-reported diseases was obtained from patient records. Eleven different proteins were measured in AH using a multiplex cytokine assay (AYOXXA Biosystems, Cologne, Germany), including IL- 6, IL-8, angiopoietin 2, C-reactive protein (CRP), vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor BB, placental growth factor, CXCL12, CXCL13, and CXCL10. Statistical analysis was performed using R and included nonparametrical testing, linear regression, and k-means clustering.
Higher nuclear LOCS grades correlated with increased levels of CCL2 (360 vs. 387 vs. 517 pg/mL, P < 0.001), VEGF-A (270 vs. 292 vs. 390 pg/mL, P = 0.012), IL-8 (3.1 vs. 4.2 vs. 5.7 pg/mL, P = 0.018), and CXCL10 (52 vs. 61 vs. 90 pg/mL, P = 0.003). No associations were observed for cortical and subcapsular cataracts. Thicker lenses were associated with significantly increased levels of CRP, CXCL10, CXCL12, IL-6, IL-8, and VEGF-A.
The cytokine profile of AH varies based on cataract grading and lens thickness. In highly dense nuclear cataracts, CCL2, VEGF-A, IL-8, and CXCL10 were elevated.
研究白内障患者房水中的细胞因子谱是否因白内障类型和严重程度而异。
本前瞻性研究纳入了 397 名患者（397 只眼），平均年龄为 76 岁（范围 30-94 岁），均接受标准的小切口超声乳化白内障吸除术。采用 LOCS III 系统对白内障进行分级：轻度（≤3）、中度（3.5-5）和重度（≥5）。使用 IOL master 700（德国蔡司 Oberkochen）进行生物测量，以区分厚晶状体（>4.5mm）和薄晶状体。从患者病历中获取年龄、性别和自报疾病等信息。使用 AYOOXA Biosystems（德国科隆）的多重细胞因子检测试剂盒（multiplex cytokine assay）在房水中测量了 11 种不同的蛋白，包括白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、血管生成素 2（angiopoietin 2）、C 反应蛋白（CRP）、血管内皮生长因子 A（vascular endothelial growth factor A，VEGF-A）、血小板衍生生长因子 BB（platelet-derived growth factor BB）、胎盘生长因子（placental growth factor）、CXCL12、CXCL13 和 CXCL10。统计分析使用 R 语言进行，包括非参数检验、线性回归和 k-均值聚类。
较高的核 LOCS 分级与 CCL2（360 与 387 与 517pg/mL，P<0.001）、VEGF-A（270 与 292 与 390pg/mL，P=0.012）、IL-8（3.1 与 4.2 与 5.7pg/mL，P=0.018）和 CXCL10（52 与 61 与 90pg/mL，P=0.003）水平的升高相关。皮质性白内障和后囊下白内障与细胞因子无相关性。较厚的晶状体与 CRP、CXCL10、CXCL12、IL-6、IL-8 和 VEGF-A 水平显著升高相关。
房水中的细胞因子谱根据白内障分级和晶状体厚度而变化。在高度致密核性白内障中，CCL2、VEGF-A、IL-8 和 CXCL10 升高。
