Li Feng, Lv Huijie, Zhu Feng, Zhang Qiuping, Xu Qian, Ji Wenliang
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
Food Chem. 2025 Jan 15;463(Pt 2):141267. doi: 10.1016/j.foodchem.2024.141267. Epub 2024 Sep 12.
Co-extraction of multiple types of target substances is the key to achieve high throughput detection. In this work, PDA@MOF-808/PAN NFsM was prepared by co-modified polyacrylonitrile nanofiber membrane (PAN NFsM) with polydopamine (PDA) and metal-organic framework-808 (MOF-808), and its potential as a solid-phase extraction (SPE) adsorbent was investigated by using the most typical nine veterinary drug residues in eggs and chicken as model target substances. The results show that PDA@MOF-808/PAN NFsM could effectively co-extract all the target substances (adsorption efficiency ranged from 81.46 % to 96.78 %), and had good capability of sample matrix purification (matrix effect was lower than -15.26 %), so a new SPE method has been established. Combined with UPLC-MS/MS, the detection limits were 0.3 to 3.1 μg/kg, the recoveries were between 71.02 % and 106.48 %, and the relative standard deviations were lower than 12.03 %, indicating that the method has considerable good sensitivity, accuracy and precision.
多种目标物质的共萃取是实现高通量检测的关键。在本工作中，通过用聚多巴胺（PDA）和金属有机框架-808（MOF-808）对聚丙烯腈纳米纤维膜（PAN NFsM）进行共改性制备了PDA@MOF-808/PAN NFsM，并以鸡蛋和鸡肉中最典型的9种兽药残留作为模型目标物质研究了其作为固相萃取（SPE）吸附剂的潜力。结果表明，PDA@MOF-808/PAN NFsM能够有效地共萃取所有目标物质（吸附效率为81.46%至96.78%），并且具有良好的样品基质净化能力（基质效应低于-15.26%），因此建立了一种新的SPE方法。结合超高效液相色谱-串联质谱（UPLC-MS/MS），检测限为0.3至3.1μg/kg，回收率在71.02%至106.48%之间，相对标准偏差低于12.03%，表明该方法具有相当好的灵敏度、准确度和精密度。
