CAS Key Laboratory of High Magnetic Field and Iron Beam Physical Biology, Institute of Intelligent Machines, Hefei Institute of Physical Sciences, Chinese Academy of Sciences, Hefei, 230031, China; Science Island Branch of Graduate School, University of Science and Technology of China, Hefei, 230026, China.
CAS Key Laboratory of High Magnetic Field and Iron Beam Physical Biology, Institute of Intelligent Machines, Hefei Institute of Physical Sciences, Chinese Academy of Sciences, Hefei, 230031, China; Science Island Branch of Graduate School, University of Science and Technology of China, Hefei, 230026, China.
Biosens Bioelectron. 2025 Jan 1;267:116784. doi: 10.1016/j.bios.2024.116784. Epub 2024 Sep 14.
Nanozymes are potential candidates for constructing sensors due to their adjustable activity, high stability, and high cost-effectiveness. However, due to the lack of reasonable means, designing and preparing efficient nanozymes remains challenging. Herein, inspired by the property of natural laccase, we applied the novel and facile low-temperature plasma (LTP) technology to fabricate a series of different base-ligand Cu metal organic framework (MOF) nanozymes (namely, A-Cu, G-Cu, C-Cu and T-Cu nanozymes) with laccase-like activity successfully. Owing to the different catalytic capacities of four types of base-Cu-MOF nanozymes in the response to five common effective bioactive substances, we constructed the nanozyme-encoded array sensor for the identification of different bioactive compounds. As a result, the four-channel colorimetric sensor array was constructed, in which four laccase-like nanozymes were utilized as the sensing units, achieving high-throughput, high-sensitivity and rapid detection/identification of five common bioactive compounds in the concentration range of 1.5-150 μg mL through different color output patterns. It is worth noting that the as-prepared sensor array can successfully distinguish the natural bioactive compounds in a variety of real samples. Furthermore, with the assistance of smartphones, we also designed a portable smart sensing approach for detecting the bioactive compounds effectively in food. This study has therefore not only provided an effective way for preparation highly effectively nanozymes, but also established a new sensing platform for intelligent sensing of bioactive components in food.
纳米酶由于其可调的活性、高稳定性和高性价比，是构建传感器的潜在候选者。然而，由于缺乏合理的手段，设计和制备高效的纳米酶仍然具有挑战性。在此，受天然漆酶性质的启发，我们应用新颖且简便的低温等离子体（LTP）技术成功制备了一系列具有漆酶样活性的不同基底配体 Cu 金属有机骨架（MOF）纳米酶（即 A-Cu、G-Cu、C-Cu 和 T-Cu 纳米酶）。由于四种基底-Cu-MOF 纳米酶在响应五种常见有效生物活性物质时的催化能力不同，我们构建了纳米酶编码的阵列传感器用于识别不同的生物活性化合物。结果，构建了一个四通道比色传感器阵列，其中四个漆酶样纳米酶作为传感单元，通过不同的颜色输出模式，在 1.5-150μg mL 的浓度范围内实现了对五种常见生物活性化合物的高通量、高灵敏度和快速检测/识别。值得注意的是，所制备的传感器阵列可以成功区分各种实际样品中的天然生物活性化合物。此外，借助智能手机，我们还设计了一种用于有效检测食品中生物活性化合物的便携式智能传感方法。因此，本研究不仅为制备高效纳米酶提供了一种有效方法，而且还建立了用于食品中生物活性成分智能传感的新传感平台。
