鸭肠炎病毒LORF4基因是一个晚期基因，对病毒在体外的复制并非必需。

Duck enteritis virus LORF4 gene is a late gene and nonessential for virus replication in vitro.

作者信息

Huang Jie, Wang Mingshu, Cheng Anchun, Yang Qiao, Tian Bin, Wu Ying, Ou Xumin, Sun Di, He Yu, Wu Zhen, Zhao Xinxin, Zhang Shaqiu, Huang Juan, Zhu Dekang, Jia Renyong, Liu Mafeng, Chen Shun

机构信息

Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China; Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

出版信息

Poult Sci. 2024 Dec;103(12):104275. doi: 10.1016/j.psj.2024.104275. Epub 2024 Aug 31.

DOI:10.1016/j.psj.2024.104275

PMID:39288717

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11421319/

Abstract

Duck enteritis virus (DEV) is an avian alpha-herpesvirus that primarily causes an acute and highly contagious infectious disease of ducks. The LORF4 gene is one of the specific genes of DEV, with limited reports on its biological characteristics and functions. This study investigates the basic biological properties of LORF4 protein (pLORF4). The results show that DEV LORF4 is a late gene mainly localized in the cytoplasm of DEV-infected DEF. To explore the role of pLORF4 in the DEV replication life cycle, a recombinant virus lacking pLORF4 expression was constructed. The results showed that pLORF4 is not essential for virus replication and does not affect virus adsorption, assembly and release, it plays a positive role in virus invasion and DNA replication. In summary, this study provides a foundation for further research on the function of the LORF4 gene.

摘要

鸭肠炎病毒（DEV）是一种禽α疱疹病毒，主要引起鸭的急性和高度传染性疾病。LORF4基因是DEV的特异性基因之一，关于其生物学特性和功能的报道有限。本研究对LORF4蛋白（pLORF4）的基本生物学特性进行了研究。结果表明，DEV LORF4是一个晚期基因，主要定位于DEV感染的鸭胚成纤维细胞（DEF）的细胞质中。为了探究pLORF4在DEV复制生命周期中的作用，构建了一种缺乏pLORF4表达的重组病毒。结果显示，pLORF4对病毒复制不是必需的，不影响病毒的吸附、组装和释放，但在病毒入侵和DNA复制中发挥积极作用。总之，本研究为进一步研究LORF4基因的功能奠定了基础。