Scholpp Anna-Catharina, Schilbert Hanna Marie, Viehöver Prisca, Weisshaar Bernd, Beckstette Michael, Neumann Judith Martha, Bednarz Hanna, Niehaus Karsten
Metabolome- and Proteome Research, Faculty of Biology & CeBiTec, Bielefeld University, Bielefeld, Germany.
Genetics and Genomics of Plants, Faculty of Biology, CeBiTec, Bielefeld University, Bielefeld, Germany.
Front Plant Sci. 2024 Sep 3;15:1412189. doi: 10.3389/fpls.2024.1412189. eCollection 2024.
, also knowen as the Japanese tea hortensia, is known for its sweet taste and health properties of bevarages produced from this plant. The 3,4-dihydroisocoumarins, hydrangenol and phyllodulcin harbour a variety of biological activities and pharmacological properties. Therefore, a detailed understanding of dihydroisocoumarin biosynthesis in is of major interest. Their biosynthesis is assumed to be enhanced by elicitors and mediated by polyketide synthases like in cases of phenylpropanoid derived phytoalexins. A transcriptome assembly of leaves and roots from the aluminium chloride treatment group versus the control group alongside with annotation was generated. Secondary plant metabolites were analysed by LC-MS. It revealed that a terpene synthase and a triterpenoid synthase gene as well as lignin biosynthesis encoding genes were upregulated in roots. Many genes for transporters, glycosyl, and other transferases as well as glycosylases were found to be differentially expressed in both organs. As no differentially expressed polyketide synthase gene homolog was found, the relative leaf and root 3,4-dihydroisocoumarin content was analysed by LC-MS measurement. Although species are known for their aluminium detoxification using phenylpropanoid-derived compounds, the levels of 3,4- dihydroisocoumarins were not enhanced. In this metabolite analysis, an organ- specific accumulation profile of hydrangenol, phyllodulcin, hydrangeic acid and their mono- and di-glycosides was figured out.
，也被称为日本茶绣球，以其甜味以及用这种植物制成的饮品的健康特性而闻名。3,4-二氢异香豆素、氢化连翘醇和卫矛醇具有多种生物活性和药理特性。因此，深入了解[植物名称]中3,4-二氢异香豆素的生物合成具有重要意义。据推测，它们的生物合成会受到诱导子的增强作用，并由聚酮合酶介导，就像苯丙烷类衍生的植物抗毒素的情况一样。生成了氯化铝处理组与对照组叶片和根部的转录组组装以及注释。通过液相色谱 - 质谱联用分析次生植物代谢产物。结果显示，根中一个萜烯合酶、一个三萜合酶基因以及编码木质素生物合成的基因上调。发现许多转运蛋白、糖基和其他转移酶以及糖苷酶的基因在两个器官中差异表达。由于未发现差异表达的聚酮合酶基因同源物，通过液相色谱 - 质谱测量分析了叶片和根中3,4-二氢异香豆素的相对含量。尽管[植物名称]物种以利用苯丙烷类衍生化合物进行铝解毒而闻名，但3,4-二氢异香豆素的水平并未提高。在这种代谢物分析中，确定了氢化连翘醇、卫矛醇、绣球酸及其单糖苷和二糖苷的器官特异性积累模式。
