Duan Lian, Hou Zhihui, Zhang Wuhua, Liang Shuang, Huangfu Minge, Zhang Jinzhu, Yang Tao, Dong Jie, Che Daidi
College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China.
Key Laboratory of Cold Region Landscape Plants and Applications, Harbin, China.
Front Plant Sci. 2024 Sep 3;15:1461322. doi: 10.3389/fpls.2024.1461322. eCollection 2024.
are a class of plant-specific transcription factors that play key roles in plant growth and stress responses. However, the mechanism by which influence adventitious root development in remains unclear. In this study, gene family in rose was identified and phylogenetically analyzed using bioinformatics analysis. A total of 381 gene members were localized on seven chromosomes except of nine members. The main -acting elements involved in hormonal, light, developmental, and abiotic stress responses were identified in the promoters of genes, suggesting their regulation by these signals. Nine genes had significant different expression during rooting process of rose. , , were positive with the formation of rose roots. RhWOX331 was positively involved in the formation of adventitious root primordia, which gene coding a transcription factor localized in the nucleus. The HOX conserved domain in the protein contributed to the self-activating activity of RhWOX331. We obtained genetically modified Arabidopsis to validate the function of . Overexpression of gene alleviated the inhibition of root length of primary roots by high concentration of IBA and NPA, and significantly increased the number of lateral roots on the primary roots, as well as the height of plants. Additionally, promoted adventitious root formation in A. thaliana and mitigated hormonal inhibition by exogenous 6-BA, NPA, and GA. The promoter contained cis-acting elements such as ABRE, Box 4 and CGTCA-motif et.al. GUS activity analysis showed that the gene acted at the cotyledon attachment site. Taken together, these studies identified a significant expansion of the gene family, inferred roles of certain branch members in adventitious root formation, elucidated the function of in adventitious root initiation, and laid the foundation for further research on the function of gene family in roses.
是一类植物特异性转录因子，在植物生长和应激反应中起关键作用。然而，它们影响不定根发育的机制在玫瑰中仍不清楚。在本研究中，利用生物信息学分析鉴定并系统发育分析了玫瑰中的基因家族。除9个成员外，共有381个基因成员定位在7条染色体上。在基因启动子中鉴定出参与激素、光、发育和非生物胁迫反应的主要顺式作用元件，表明它们受这些信号调控。9个基因在玫瑰生根过程中表达有显著差异。、、与玫瑰根的形成呈正相关。RhWOX331正向参与不定根原基的形成，其基因编码一种定位于细胞核的转录因子。蛋白质中的HOX保守结构域有助于RhWOX331的自激活活性。我们获得了转基因拟南芥以验证的功能。基因的过表达减轻了高浓度IBA和NPA对拟南芥主根根长的抑制，并显著增加了主根上侧根的数量以及拟南芥植株的高度。此外，促进了拟南芥不定根的形成，并减轻了外源6 - BA、NPA和GA对激素的抑制作用。基因启动子包含ABRE、Box 4和CGTCA - motif等顺式作用元件。GUS活性分析表明该基因在子叶附着位点起作用。综上所述，这些研究确定了基因家族的显著扩张，推断了某些分支成员在不定根形成中的作用，阐明了在不定根起始中的功能，并为进一步研究玫瑰中基因家族的功能奠定了基础。
