Huangfu Shuchen, Zheng Jie, He Jiashuai, Liao Jin, Jiang Haiping, Zhou Hua, Pan Jinghua
Department of General Surgery, the First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.
iScience. 2024 Jul 14;27(9):110494. doi: 10.1016/j.isci.2024.110494. eCollection 2024 Sep 20.
The interplay between intestinal barrier degradation and trace element insufficiency worsens inflammatory bowel disease (IBD). Selenium (se) is essential for glutathione peroxidase 4 (GPX4) synthesis, which protects against intestinal epithelial cell injury in IBD. However, malnutrition and malabsorption limit the availability of dietary selenium. This study investigated the protective effects of naturally occurring seleno-amino acids on the intestinal barrier in an IBD animal model by promoting GPX4 synthesis. L-se-methylselenocystine (seMc) supplementation reversed decreased GPX4 expression levels, alleviated glutathione depletion and scavenged reactive oxygen species . , enteral nutrition combined with seMc protected the intestinal barrier and alleviated IBD-related symptoms by inhibiting ferroptosis and reversing lipid peroxidation in epithelial cells while reducing immune cell infiltration. Our findings suggest that seleno-amino acid-based nutritional formulations may provide a basis for nutritional support to alleviate complex cycles between intestinal barrier damage and malnutrition in IBD patients.
肠道屏障降解与微量元素不足之间的相互作用会使炎症性肠病（IBD）恶化。硒（Se）对于谷胱甘肽过氧化物酶4（GPX4）的合成至关重要，GPX4可保护IBD患者的肠道上皮细胞免受损伤。然而，营养不良和吸收不良限制了膳食硒的可利用性。本研究通过促进GPX4合成，探讨了天然存在的硒氨基酸对IBD动物模型肠道屏障的保护作用。补充L-硒甲基硒代半胱氨酸（SeMc）可逆转GPX4表达水平的降低，减轻谷胱甘肽耗竭并清除活性氧。此外，肠内营养联合SeMc通过抑制铁死亡和逆转上皮细胞中的脂质过氧化，同时减少免疫细胞浸润，保护了肠道屏障并减轻了IBD相关症状。我们的研究结果表明，基于硒氨基酸的营养制剂可能为营养支持提供依据，以缓解IBD患者肠道屏障损伤与营养不良之间的复杂循环。
