Beard C A, Wrightsman R A, Manning J E
Mol Biochem Parasitol. 1985 Aug;16(2):199-212. doi: 10.1016/0166-6851(85)90087-8.
The surface polypeptides of epimastigotes and tissue culture-derived trypomastigotes of Trypanosoma cruzi have been isolated free of most cytosolic components by use of the 2-iminobiotin-avidin interaction. Polypeptides of the trypomastigote stage obtained by this technique are recognized by serum antibodies from Chagasic patients and T. cruzi-infected mice. These polypeptides have been used as the detecting antigen for the identification of hybridoma cells producing monoclonal antibodies against the surface proteins of the trypomastigote stage of T. cruzi. These experiments document a practical approach for obtaining T. cruzi surface proteins in sufficient quantity and purity for use in immunological studies.
通过利用2-亚氨基生物素-抗生物素蛋白相互作用，已从克氏锥虫的上鞭毛体和组织培养衍生的锥鞭毛体中分离出表面多肽，且大部分胞质成分已被去除。用该技术获得的锥鞭毛体阶段的多肽可被恰加斯病患者和感染克氏锥虫的小鼠血清抗体识别。这些多肽已被用作检测抗原，用于鉴定产生针对克氏锥虫锥鞭毛体阶段表面蛋白的单克隆抗体的杂交瘤细胞。这些实验证明了一种实用方法，可获得足够数量和纯度的克氏锥虫表面蛋白，用于免疫学研究。
