Department of Stomatology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.
SanYe Pediatric Dental Clinic, Hangzhou Stomatology Hospital, Hangzhou, Zhejiang Province, China.
J Mech Behav Biomed Mater. 2024 Dec;160:106750. doi: 10.1016/j.jmbbm.2024.106750. Epub 2024 Sep 16.
Proanthocyanidin (PA) has demonstrated promise as a dental biomodifier for maintaining dentin collagen integrity, yet there is limited evidence regarding its efficacy in dentin repair. The aim of this study was to investigate the effect of PA on dentin remineralization through the polymer induced liquid precursor (PILP) process, as well as to assess the mechanical properties of the restored dentin. Demineralized dentin was treated with a PA-contained remineralization medium, resulting in the formation of PA-amorphous calcium phosphate (ACP) nanoparticles via the PILP process. The kinetics and microstructure of remineralized dentin were examined through the use of Fourier transform infrared spectroscopy(FTIR), attenuated total reflectance-FTIR, scanning electron microscopy, transmission electron microscopy. The results showed that the application of PA facilitated the process of dentin remineralization, achieving completion within 48 h, demonstrating a notable reduction in time required. Following remineralization, the mechanical properties of the dentin exhibited an elastic modulus of 15.89 ± 1.70 GPa and a hardness of 0.47 ± 0.08 GPa, which were similar to those of natural dentin. These findings suggest that combining PA with the PILP process can promote dentin remineralization and improve its mechanical properties, offering a promising new approach for dentin repair in clinical practice.
原花青素（PA）作为一种维持牙本质胶原完整性的牙科生物调节剂具有很大的应用潜力，但关于其在牙本质修复中的功效的证据有限。本研究旨在通过聚合物诱导的液体前体（PILP）过程研究 PA 对牙本质再矿化的影响，并评估修复牙本质的机械性能。用含有 PA 的再矿化介质处理脱矿牙本质，通过 PILP 过程形成 PA-无定形磷酸钙（ACP）纳米颗粒。通过傅里叶变换红外光谱（FTIR）、衰减全反射-FTIR、扫描电子显微镜、透射电子显微镜研究了再矿化牙本质的动力学和微观结构。结果表明，PA 的应用促进了牙本质再矿化过程，在 48 小时内完成，显著缩短了所需时间。再矿化后，牙本质的力学性能表现出 15.89±1.70GPa 的弹性模量和 0.47±0.08GPa 的硬度，与天然牙本质相似。这些发现表明，将 PA 与 PILP 工艺相结合可以促进牙本质再矿化并改善其机械性能，为临床牙本质修复提供了一种有前途的新方法。
