Behera Sandesh, Singh Deepika, Mohapatra Sonali, Behera Bikash Chandra, Thatoi Hrudayanath
Department of Biotechnology, Maharaja Sriram Chandra BhanjaDeo University, Baripada 757003, Odisha, India.
Department of Physics, Indian Institute of Technology Roorkee, Uttarakhand 247667, India.
Int J Biol Macromol. 2024 Sep 16;280(Pt 1):135738. doi: 10.1016/j.ijbiomac.2024.135738.
Bioactive substances are utilized to treat a variety of diseases. Green lignin-mediated silver nanoparticles (L-Ag-NPs) have significant promise as a building block in the production of bio-renovation materials. The work optimized organic acid extraction to remove lignin from residual fermented hybrid Napier grass byproducts. We subsequently produced L-Ag-NPs. FTIR, XRD, DLS, and STEM characterized the sample. L-Ag-NPs were tested for antioxidant activity with the DPPH, DMPD, FRAP, and ABTS assays, as well as antibacterial activities. Antimicrobial activity was evaluated using four pathogenic bacteria (Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli). In contrast, cytotoxicity and ROS production assays were carried out using the HeLa cell line. The findings showed that L-Ag-NPs had high antioxidant efficiency. For each bacteria isolate, the antimicrobial activity showed favorable growth inhibition, with significant variations in L-Ag-NPs. L-Ag-NPs were reported to have an IC of 43.61 g/mL in the cytotoxicity test, and a significant increase in ROS generation was seen. In conclusion, L-Ag NPs have an excellent prospect in the pharmaceutical and biomedical industries and can be a dependable and environmentally safe material for their potential use.
生物活性物质被用于治疗多种疾病。绿色木质素介导的银纳米颗粒（L-Ag-NPs）作为生物修复材料生产中的一个组成部分具有巨大潜力。这项工作优化了从残留发酵杂交狼尾草副产品中去除木质素的有机酸提取方法。随后我们制备了L-Ag-NPs。通过傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、动态光散射（DLS）和扫描透射电子显微镜（STEM）对样品进行了表征。采用二苯基苦味酰基自由基（DPPH）、二甲基吡啶鎓二氯化物（DMPD）、铁离子还原抗氧化能力（FRAP）和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）法对L-Ag-NPs的抗氧化活性以及抗菌活性进行了测试。使用四种病原菌（肺炎克雷伯菌、铜绿假单胞菌、金黄色葡萄球菌和大肠杆菌）评估抗菌活性。相比之下，使用人宫颈癌细胞系（HeLa细胞系）进行了细胞毒性和活性氧（ROS）产生测定。结果表明，L-Ag-NPs具有较高的抗氧化效率。对于每种分离出的细菌，抗菌活性均表现出良好的生长抑制作用，L-Ag-NPs存在显著差异。据报道，在细胞毒性试验中L-Ag-NPs的半数抑制浓度（IC）为43.61 μg/mL，并且观察到ROS生成显著增加。总之，L-Ag NPs在制药和生物医学行业具有良好的前景，因其潜在用途可成为一种可靠且对环境安全的材料。
