Hou Chunyan, Li Weiyu, Li Yaoxiang, Ma Junfeng
Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, 20007, USA.
Department of Applied Mathematics and Statistics, Johns Hopkins University, Baltimore, MD, 21218, USA.
Anal Bioanal Chem. 2025 Feb;417(5):895-905. doi: 10.1007/s00216-024-05531-2. Epub 2024 Sep 18.
As a post-translational modification, protein glycosylation is critical in health and disease. O-Linked β-N-acetylglucosamine (O-GlcNAc) modification (O-GlcNAcylation), as an intracellular monosaccharide modification on proteins, was discovered 40 years ago. Thanks to technological advances, the physiological and pathological significance of O-GlcNAcylation has been gradually revealed and widely appreciated, especially in recent years. O-GlcNAc informatics has been quickly evolving. Clearly, O-GlcNAc informatics tools have not only facilitated O-GlcNAc functional studies, but also provided us a unique perspective on protein O-GlcNAcylation. In this article, we review O-GlcNAc-focused software tools and servers that have been developed for O-GlcNAc research over the past four decades. Specifically, we will (1) survey bioinformatics tools that have facilitated O-GlcNAc proteomics data analysis, (2) introduce databases/servers for O-GlcNAc proteins/sites that have been experimentally identified by individual research labs, (3) describe software tools that have been developed to predict O-GlcNAc sites, and (4) introduce platforms cataloging proteins that interact with the O-GlcNAc cycling enzymes (i.e., O-GlcNAc transferase and O-GlcNAcase). We hope these resources will provide useful information to both experienced researchers and new incomers to the O-GlcNAc field. We anticipate that this review provides a framework to stimulate the future development of more sophisticated informatic tools for O-GlcNAc research.
作为一种翻译后修饰，蛋白质糖基化在健康和疾病中至关重要。O-连接的β-N-乙酰葡糖胺（O-GlcNAc）修饰（O-GlcNAc化）作为蛋白质上的一种细胞内单糖修饰，于40年前被发现。得益于技术进步，O-GlcNAc化的生理和病理意义已逐渐被揭示并得到广泛认可，尤其是在近年来。O-GlcNAc信息学一直在快速发展。显然，O-GlcNAc信息学工具不仅促进了O-GlcNAc功能研究，还为我们提供了关于蛋白质O-GlcNAc化的独特视角。在本文中，我们回顾了在过去四十年中为O-GlcNAc研究而开发的以O-GlcNAc为重点的软件工具和服务器。具体而言，我们将（1）调查有助于O-GlcNAc蛋白质组学数据分析的生物信息学工具，（2）介绍由各个研究实验室通过实验鉴定的O-GlcNAc蛋白质/位点的数据库/服务器，（3）描述为预测O-GlcNAc位点而开发的软件工具，以及（4）介绍对与O-GlcNAc循环酶（即O-GlcNAc转移酶和O-GlcNAcase）相互作用的蛋白质进行编目的平台。我们希望这些资源能为O-GlcNAc领域的经验丰富的研究人员和新手都提供有用的信息。我们预计这篇综述提供了一个框架，以刺激未来为O-GlcNAc研究开发更复杂的信息学工具。
