Gong Baoying, Chen Jiayi, Yu Haizhen, Li Shizhen
Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
Department of Clinical Laboratory, Jiading Branch of Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 201803, China.
Int Urol Nephrol. 2025 Feb;57(2):603-611. doi: 10.1007/s11255-024-04209-z. Epub 2024 Sep 19.
Sepsis often leads to a cluster of life-threatening symptoms affecting multiple organ systems. Among the organs most vulnerable to damage in this state is the kidney; those afflicted with severe sepsis frequently encounter acute kidney injury (AKI). The study was to investigate the diagnostic role of miR-625-5p in sepsis and sepsis-induced acute kidney injury (SI-AKI) and predict the possible pathways of miR-625-5p involved in SI-AKI by bioinformatics method.
RT-qPCR was used to detect the level of miR-625-5p, and the diagnostic value of miR-625-5p was analyzed using ROC curve. The proliferation and the concentration of inflammatory factors of HK-2 cells induced by LPS were detected by CCK-8 and ELISA. The pathway of miR-625-5p involved in SI-AKI was analyzed by bioinformatics method.
The miR-625-5p expression was downregulated in sepsis as well as SI-AKI and has predictive value for sepsis as well as SI-AKI. In addition, miR-625-5p promoted LPS-induced cell proliferation and inhibited the levels of inflammatory cytokines.
miR-625-5p may be a diagnostic biomarker for SI-AKI.
脓毒症常导致一系列危及生命的症状，影响多个器官系统。在此状态下最易受损的器官之一是肾脏；患有严重脓毒症的患者经常会出现急性肾损伤（AKI）。本研究旨在探讨miR-625-5p在脓毒症及脓毒症诱导的急性肾损伤（SI-AKI）中的诊断作用，并通过生物信息学方法预测miR-625-5p参与SI-AKI的可能途径。
采用RT-qPCR检测miR-625-5p水平，利用ROC曲线分析miR-625-5p的诊断价值。通过CCK-8和ELISA检测LPS诱导的HK-2细胞的增殖及炎症因子浓度。采用生物信息学方法分析miR-625-5p参与SI-AKI的途径。
miR-625-5p在脓毒症及SI-AKI中表达下调，对脓毒症及SI-AKI具有预测价值。此外，miR-625-5p促进LPS诱导的细胞增殖并抑制炎症细胞因子水平。
miR-625-5p可能是SI-AKI的诊断生物标志物。
