Wu Yan-Xia, Yin Shuo, Song Shan-Shan, Liu Xiang, Deng Yu-Xuan, Lu Xue-Jing
Eye School of Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China.
Ineye Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610084, Sichuan Province, China.
Int J Ophthalmol. 2024 Sep 18;17(9):1599-1605. doi: 10.18240/ijo.2024.09.04. eCollection 2024.
To investigate the antioxidant protective effect of glycopeptide (LbGP) pretreatment on retinal ischemia-reperfusion (I/R) injury (RIRI) in rats.
RIRI was induced in Sprague Dawley rats through anterior chamber perfusion, and pretreatment involved administering LbGP gavage for 7d. After 24h of reperfusion, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and creatinine (CREA) levels, retinal structure, expression of Caspase-3 and Caspase-8, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) in the retina were measured.
The pretreatment with LbGP effectively protected the retina and retinal tissue from edema and inflammation in the ganglion cell layer (GCL) and nerve fiber layer (NFL) of rats subjected to RIRI, as shown by light microscopy and optical coherence tomography (OCT). Serum AST was higher in the model group than in the blank group (=0.042), but no difference was found in ALT, AST, and CREA across the LbGP groups and model group. Caspase-3 expression was higher in the model group than in the blank group (=0.006), but no difference was found among LbGP groups and the model group. Caspase-8 expression was higher in the model group than in the blank group (=0.000), and lower in the 400 mg/kg LbGP group than in the model group (=0.016). SOD activity was lower in the model group than in the blank group (=0.001), and the decrease was slower in the 400 mg/kg LbGP group than in the model group (=0.003). MDA content was higher in the model group than in the blank group (=0.001), and lower in the 400 mg/kg LbGP group than in the model group (=0.016). The pretreatment with LbGP did not result in any observed liver or renal toxicity in the model.
LbGP pretreatment exhibits dose-dependent anti-inflammatory, and antioxidative effects by reducing Caspase-8 expression, preventing declines of SOD activity, and decreasing MDA content in the RIRI rat model.
探讨糖肽（LbGP）预处理对大鼠视网膜缺血再灌注（I/R）损伤（RIRI）的抗氧化保护作用。
通过前房灌注诱导Sprague Dawley大鼠发生RIRI，预处理为连续7天经口灌胃给予LbGP。再灌注24小时后，检测血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和肌酐（CREA）水平、视网膜结构、半胱天冬酶-3（Caspase-3）和半胱天冬酶-8（Caspase-8）的表达、超氧化物歧化酶（SOD）活性以及视网膜中的丙二醛（MDA）。
光学显微镜和光学相干断层扫描（OCT）显示，LbGP预处理有效保护了RIRI大鼠视网膜及视网膜组织，使其免受神经节细胞层（GCL）和神经纤维层（NFL）的水肿和炎症影响。模型组血清AST高于空白组（P=0.042），但LbGP各剂量组与模型组之间ALT、AST和CREA无差异。模型组Caspase-3表达高于空白组（P=0.006），但LbGP各剂量组与模型组之间无差异。模型组Caspase-8表达高于空白组（P=0.000），400mg/kg LbGP组低于模型组（P=0.016）。模型组SOD活性低于空白组（P=0.001），400mg/kg LbGP组下降速度慢于模型组（P=0.003）。模型组MDA含量高于空白组（P=0.001），400mg/kg LbGP组低于模型组（P=0.016）。LbGP预处理在模型中未观察到任何肝脏或肾脏毒性。
在RIRI大鼠模型中，LbGP预处理通过降低Caspase-8表达、防止SOD活性下降和降低MDA含量，呈现出剂量依赖性的抗炎和抗氧化作用。
