Ursic D
Biochem Biophys Res Commun. 1985 Aug 30;131(1):152-9. doi: 10.1016/0006-291x(85)91783-8.
We investigated whether the same or different T-DNA insertions occur every time Agrobacterium tumefaciens, the octopine type strain pTi 15955 strr, infects genetically identical sunflower plants. Eight newly established crown gall tissue culture lines were analyzed for their T-DNA content. Our data showed that all isogenic crown gall callus DNA produced distinct hybridization patterns. These eight patterns were also different from three standard lines included for comparison. In addition, all the tumor lines analyzed produced octopine, albeit in different quantities, and five produced agropine and mannopine. We concluded, that each A. tumefaciens crown gall tissue line derived from isogenic sunflower plants contained a distinct insertion pattern of T-DNA. Possible causes and reasons for this diversity will be discussed.
我们研究了每次根癌土壤杆菌（章鱼碱型菌株pTi 15955 strr）感染基因相同的向日葵植株时，是否会发生相同或不同的T-DNA插入。对8个新建立的冠瘿组织培养系的T-DNA含量进行了分析。我们的数据表明，所有同基因冠瘿愈伤组织DNA产生了不同的杂交模式。这8种模式也与用于比较的3个标准系不同。此外，所有分析的肿瘤系都产生了章鱼碱，尽管数量不同，并且有5个产生了农杆碱和甘露碱。我们得出结论，源自同基因向日葵植株的每个根癌土壤杆菌冠瘿组织系都含有独特的T-DNA插入模式。将讨论这种多样性的可能原因。
