Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima Str. 10, 10-748, Olsztyn, Poland.
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima Str. 10, 10-748, Olsztyn, Poland.
Talanta. 2025 Jan 1;281:126870. doi: 10.1016/j.talanta.2024.126870. Epub 2024 Sep 17.
The interest in biosensors technology has been constantly growing over the last few years. It is still the biggest challenge to design biosensors able to detect two or more analytes in a single measurement. Electrochemical methods are frequently used for this purpose, mainly due to the possibility of applying two or more different redox labels characterized by independent and distinguished electrochemical signals. In addition to antibodies, nucleic acids (aptamers) have been increasingly used as bioreceptors in the construction of such sensors. Within this review paper, we have collected the examples of electrochemical immuno- and geno(apta)sensors for simultaneous detection of multiple analytes. Based on many published literature examples, we have emphasized the recent application of multiplexed platforms for detection of cancer biomarkers. It has allowed us to compare the progress in design strategies, including novel nanomaterials and amplification of signals, to get as low as possible limits of detection. We have focused on multi-electrode and multi-label strategies based on redox-active labels, such as ferrocene, anthraquinone, methylene blue, thionine, hemin and quantum dots, or metal ions such as Ag, Pb, Cd, Zn, Cu and others. We have finally discussed the possible way of development, challenges and prospects in the area of multianalyte electrochemical immuno- and geno(apta)sensors.
近年来，生物传感器技术的研究兴趣不断增长。设计能够在单次测量中检测两种或更多种分析物的生物传感器仍然是最大的挑战。电化学方法常用于此目的，主要是因为可以应用两种或更多种具有独立且不同电化学信号的不同氧化还原标记。除了抗体外，核酸（适配体）也越来越多地被用作此类传感器构建中的生物受体。在这篇综述论文中，我们收集了用于同时检测多种分析物的电化学免疫和基因（适配体）传感器的例子。基于许多已发表的文献实例，我们强调了用于检测癌症生物标志物的多重平台的最新应用。这使我们能够比较设计策略的进展，包括新型纳米材料和信号放大，以获得尽可能低的检测限。我们专注于基于氧化还原活性标记（如二茂铁、蒽醌、亚甲蓝、噻吩、血红素和量子点）或金属离子（如 Ag、Pb、Cd、Zn、Cu 等）的多电极和多标记策略。最后，我们讨论了多分析物电化学免疫和基因（适配体）传感器领域的可能发展方向、挑战和前景。
