School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China.
School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China; Key Laboratory of Haikou Trauma, Key Laboratory of Hainan Trauma and Disaster Rescue, Key Laboratory of Emergency and Trauma, Ministry of Education, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou 571199, Hainan, China.
Talanta. 2025 Jan 1;281:126914. doi: 10.1016/j.talanta.2024.126914. Epub 2024 Sep 18.
As widely used antibiotics, tetracycline residues exist in food and environmental media, which pose certain hidden dangers and negative effects on public health. Therefore, the sensing and discrimination of tetracycline analogs (TCs) have great significance for improving food safety and preventing environmental pollution. Herein, a 7-hydroxycoumarin-3-carboxylic acid-embedded Eu-MOF (HC@Eu-MOF) material was constructed and then developed for the detection of TCs. Upon addition of TCs, the synthesized sensor displays opposite fluorescence changes at two different wavelengths due to the simultaneous presence of the inner filter effect (IFE) and the antenna effect (AE), and achieves a stable ratio signal response within 90 s. In addition, six important tetracycline analogs, including chlortetracycline (CTC), oxytetracycline (OTC), tetracycline (TC), metacycline (MC), doxycycline (DC) and demeclocycline (DMC) can be discriminated with 100 % accuracy through the principal component analysis even in extremely complicated mixtures. Further, a smartphone-assisted portable device was applied for visual sensing of TCs. The as-developed platform possessed the characteristics of simple synthesis, fast response, high sensitivity, and high stability, which further lays a further foundation for the on-site visual detection and discrimination of TCs in real samples.
作为广泛使用的抗生素，四环素类抗生素残留存在于食品和环境介质中，对公众健康构成一定的潜在危害和负面影响。因此，四环素类似物（TCs）的传感和识别对于提高食品安全和防止环境污染具有重要意义。本文构建了一种 7-羟基香豆素-3-羧酸嵌入的 Eu-MOF（HC@Eu-MOF）材料，并将其用于 TCs 的检测。在加入 TCs 后，由于内滤效应（IFE）和天线效应（AE）的同时存在，合成的传感器在两个不同波长处显示出相反的荧光变化，并且在 90s 内实现了稳定的比信号响应。此外，通过主成分分析，可以准确地区分六种重要的四环素类似物，包括金霉素（CTC）、土霉素（OTC）、四环素（TC）、米诺环素（MC）、强力霉素（DC）和去甲金霉素（DMC），即使在极其复杂的混合物中也能达到 100%的准确率。进一步地，应用智能手机辅助的便携式设备进行 TCs 的可视化传感。所开发的平台具有合成简单、响应快速、灵敏度高、稳定性好的特点，为现场可视化检测和实际样品中 TCs 的识别进一步奠定了基础。
