在活体草履虫细胞表面膜标记过程中环糊精-丹磺酰氯复合物的内化

Internalization of cycloheptaamylose-dansyl chloride complex during labelling of surface membrane in living Paramecium aurelia cells.

作者信息

Giordano P A, Wyroba E, Bottiroli G

出版信息

Basic Appl Histochem. 1985;29(2):121-33.

Abstract

Internalization of cycloheptaamylose-dansyl chloride complex during surface labelling of living long-term starved Paramecium aurelia cells has been observed. This process may be inhibited by pretreatment of the ciliates with dichloroisoproterenol. Uptake of cycloheptaamylose-dansyl chloride may be visualized only after UV preirradiation: the appearance of orange-fluorescing vacuoles of diameter 2.3-4.5 micron may then be observed. Microspectrographic analysis performed on the cells and dansyl derivatives indicates that this fluorescence is produced by a photochemical reaction of dansyl chloride - released from CDC complex inside the digestive vacuoles-under the influence of UV irradiation.

摘要

已观察到在长期饥饿的活体草履虫细胞表面标记过程中，环庚糖淀粉酶 - 丹磺酰氯复合物的内化现象。用二氯异丙肾上腺素预处理纤毛虫可抑制这一过程。环庚糖淀粉酶 - 丹磺酰氯的摄取只有在紫外线预照射后才能显现：随后可观察到直径为2.3 - 4.5微米的橙色荧光液泡的出现。对细胞和丹磺酰衍生物进行的显微光谱分析表明，这种荧光是由消化液泡内从环庚糖淀粉酶 - 丹磺酰氯复合物释放的丹磺酰氯在紫外线照射影响下发生的光化学反应产生的。