Nano-phytosome loaded Retama raetam extract/colistin: antibacterial, antioxidant activities and in vivo lipopolysaccharide-induced-neurotoxicity inhibition.

Antibiotics are misused nowadays, leading to the prevalence of antibiotic resistant bacterial strains; causing the world to move towards natural medicine. Retama raetam had wide medicinal use. In the present study, R. raetam ethanolic extract proved to be active against Pseudomonas aeruginosa with MIC values ranged from 15.62 to 250 µg/ml. Antioxidant analysis showed that the extract had high scavenging activity reached 92.40%. GC/MS analysis revealed that Sparteine and Tributyl acetylcitrate represent the extract major components. Furthermore, the combination between Retama raetam extract and colistin showed a synergistic effect. Moreover, nano-phytosome was designated and optimized to encapsulate Retama raetam extract/Colistin. Nano-phytosome characterized by particle size, Zeta potential, polydispersity index and Entrapment efficiency percentage of 16.92-32.85 nm, -30.40 mV, 0.26 and 89% respectively. The antibacterial activity of the prepared nano-phytosome formula against P. aeruginosa showed promising MIC, MBC, MIC index, and IZ diameter reaching 7.81, 15.62 µg/ml, 2, and 39 mm, respectively. While TEM examination of P. aeruginosa cells treated with nano-phytosome formula revealed cell wall breakage which led to cell death. Finally, P. aeruginosa LPS was used to induce neurodegenerative disease in rat model. Rats treated with nano-phytosome formula showed normal histoarchitecture organization and the cerebral cortex was partially restored compared to control groups.