Bouzenna Hafsia, Ben Nasr Hmed, Hfaeidh Najla, Missaoui Ahlem, Giuffrè Angelo Maria, Hosni Karim, Jebahi Samira, Mejri Naceur
Laboratory of Biotechnology and Biomonitoring of the Environment and Oasis Ecosystems (LBBEEO), Faculty of Sciences of Gafsa, University of Gafsa, Gafsa, Tunisia.
Department of Life Sciences, Faculty of Sciences of Gafsa, University of Gafsa, Gafsa, Tunisia.
Front Pharmacol. 2025 Jul 31;16:1621579. doi: 10.3389/fphar.2025.1621579. eCollection 2025.
In this study, the wound healing properties of the leaf aqueous extract of (Forssk) Webb & Berthel was investigated and . The HPLC-DAD profiling of bioactive compounds allowed the identification of five phenolics including quercetin, kaempferol, naringenin, myricetin and caffeic acid. The aqueous extract has been found to significantly inhibit microbial growth in the wounded tissue contributing thereby to its cleansing. The topical application of the aqueous extract accelerated wound closure and enhanced the re-epithelialization and restoration of damaged wound skin. The wound healing activity of the which was supported by histopathological observations exceeded that of the standard wound healing cream biafine. Concomitantly, the wound-repairing action was associated with a reduced oxidative stress as revealed by the decrease of lipid peroxidation versus the activation of antioxidant enzymes SOD, CAT and GPx. study showed that naringenin and to a less extent myricetin have the highest bending capacity to the active site of pro-inflammatory cytokines TNF-α and IL-1β receptors. These results indicate that could be considered as a consolidated source of putative bioactive phenolics with excellent antioxidant, antimicrobial, anti-inflammatory and wound healing properties, offering thereby experimental and theoretical supports for its application in the treatment of burn skin wounds.
在本研究中，对（Forssk）Webb & Berthel叶水提取物的伤口愈合特性进行了研究。生物活性化合物的HPLC-DAD分析鉴定出了五种酚类物质，包括槲皮素、山奈酚、柚皮素、杨梅素和咖啡酸。已发现水提取物能显著抑制受伤组织中的微生物生长，从而有助于伤口清洁。水提取物的局部应用加速了伤口闭合，增强了受损伤口皮肤的再上皮化和修复。组织病理学观察结果支持的该提取物的伤口愈合活性超过了标准伤口愈合乳膏比亚芬。与此同时，伤口修复作用与氧化应激降低有关，这表现为脂质过氧化减少，同时抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）被激活。研究表明，柚皮素以及程度稍低的杨梅素对促炎细胞因子肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）受体的活性位点具有最高的结合能力。这些结果表明，该提取物可被视为一种可靠的推定生物活性酚类物质来源，具有优异的抗氧化、抗菌、抗炎和伤口愈合特性，从而为其在烧伤皮肤伤口治疗中的应用提供了实验和理论支持。
