Section of Pharmacognosy & Chemistry of Natural Products, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, 15771, Athens, Greece.
Department of Pharmacology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100, Burjassot, Spain.
Chem Biodivers. 2024 Nov;21(11):e202400457. doi: 10.1002/cbdv.202400457. Epub 2024 Sep 20.
Genus Stachys L. consists of approximately 365 species, distributed mainly in temperate regions. Several members of this genus are widely used in the traditional medicine of different countries worldwide. In Greece, 54 Stachys taxa are found in parts of the mainland and/or insular country. The present study focused on the phytochemical investigation of Stachys iva Griseb. and the in vitro anti-inflammatory evaluation of the isolated compounds. In total, eighteen compounds were isolated and identified from the dichloromethane-methanol extract, belonging to iridoids, flavonoids, phenylethanoid glycosides, and phenolic acids. An in vitro approach assessed the aryl hydrocarbon receptor (AhR) modulatory effects of these compounds, revealing an AhR agonistic activity of the flavonoid aglycones apigenin and cirsimaritin in HepG2 and HT29 cell lines. The present study contributes to the evidence of the traditional uses of Stachys spp. and its bioactive constituents, justifying the ethnopharmacological use as an anti-inflammatory plant genus.
属 Stachys L. 约有 365 种，主要分布于温带地区。该属的几个成员在世界不同国家的传统医学中被广泛使用。在希腊，在该国大陆和/或岛屿的部分地区发现了 54 种 Stachys 分类群。本研究专注于 Stachys iva Griseb. 的植物化学研究以及分离化合物的体外抗炎评估。从二氯甲烷-甲醇提取物中总共分离并鉴定了 18 种化合物，属于环烯醚萜、类黄酮、苯乙醇苷和酚酸。体外方法评估了这些化合物对芳烃受体 (AhR) 的调节作用，揭示了类黄酮苷元芹菜素和紫铆素在 HepG2 和 HT29 细胞系中的 AhR 激动活性。本研究为 Stachys spp. 的传统用途及其生物活性成分提供了证据，证明了作为抗炎植物属的民族药理学用途。
