Department of Forestry, Range and Wildlife Management, Islamia University of Bahawalpur, Pakistan.
Department of Zoology, The Islamia University of Bahawalpur, Pakistan.
PLoS One. 2024 Sep 20;19(9):e0306583. doi: 10.1371/journal.pone.0306583. eCollection 2024.
Acetochlor is frequently applied to various food crops in agriculture sector, and long-term exposure can cause significant endocrine-disrupting effects in exposed animals including impacts on human health. This study aimed to evaluate the effects of acetochlor on the growth, hematology, serum biochemistry, and histopathological alterations in Japanese quail. Eighty male quail were obtained and divided into four groups (A-D) and given acetochlor orally for the period of 45 days. Group A was served as the control, while groups B, C, and D received 20mg/kg, 30mg/kg, and 40mg/kg acetochlor, respectively. The study found that Japanese quail administered higher doses of acetochlor exhibited reduced frequency of crowing and foam production. The results showed that increased concentrations of acetochlor led to adverse effects on the growth parameters of Japanese quail. Hematology analysis indicated that birds exposed to higher concentrations of acetochlor experienced a significant decrease in red blood cell count, hemoglobin concentration, hematocrit (HCT), mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), along with a significant increase in white blood cell count compared to the control group. Additionally, higher concentrations of acetochlor led to a significant increase in various serological indices including urea, creatinine, alanine aminotransferase (ALT), and aspartate aminotransferase (AST), while the values of total proteins, albumin, and plasma proteins declined. The histopathology results of treated Japanese quail exposed to higher concentrations of acetochlor showed a range of pathological lesions in the testes, heart, and brain. The study concluded that even low concentrations of acetochlor can cause slight to significant changes in Japanese quail, affecting their physical, hematological, histopathological and serum biochemical parameters.
乙酰甲胺磷在农业领域常被用于各种食用作物，长期暴露于该物质可能会对暴露动物产生显著的内分泌干扰效应，包括对人类健康的影响。本研究旨在评估乙酰甲胺磷对鹌鹑生长、血液学、血清生化和组织病理学变化的影响。实验选用 80 只雄性鹌鹑，随机分为 4 组（A-D），并通过口灌服的方式给予乙酰甲胺磷 45 天。A 组为对照组，B、C 和 D 组分别灌胃 20mg/kg、30mg/kg 和 40mg/kg 剂量的乙酰甲胺磷。研究发现，高剂量乙酰甲胺磷处理组鹌鹑的鸣叫和泡沫产生频率降低。结果表明，较高浓度的乙酰甲胺磷对鹌鹑的生长参数产生了不利影响。血液学分析表明，暴露于较高浓度乙酰甲胺磷的鸟类红细胞计数、血红蛋白浓度、红细胞压积（HCT）、平均红细胞体积（MCV）和平均红细胞血红蛋白浓度（MCHC）显著降低，白细胞计数显著升高，与对照组相比。此外，较高浓度的乙酰甲胺磷导致各种血清学指标显著升高，包括尿素、肌酐、丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST），而总蛋白、白蛋白和血浆蛋白的含量下降。暴露于高浓度乙酰甲胺磷的鹌鹑的组织病理学结果显示，睾丸、心脏和大脑出现了一系列病变。研究表明，即使是低浓度的乙酰甲胺磷也会导致鹌鹑出现轻微至显著的变化，影响其生理、血液学、组织病理学和血清生化参数。
