Kan Ziwei, Chen Ye, Zhang Qianyun, Pan Luyi, Chen Aiying, Wang Ding, Wang Yi, Wan Guangming, He Bin
School of Material Science & Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
Department of Critical Care Medicine, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, China.
Colloids Surf B Biointerfaces. 2025 Jan;245:114249. doi: 10.1016/j.colsurfb.2024.114249. Epub 2024 Sep 16.
The catheters coating can be effective in reducing bloodstream infection and thrombosis, which are the major complications in blood contact catheters. However, the surface functional coating is difficult to be implemented due to the high surface stretching force from the minor-caliber. In this work, we propose a covalent bonding coating of polydopamine/titanium dioxide quantum dots (PDA/TiO QDs) on polyurethane (PU) catheters, which can fulfill a dual-function of antibacterial and antithrombosis. The PDA/TiO QDs layer was prepared by dip-coating, where the intermediate transition layer of PDA was reacted with the internal hydroxyls of PU surface by pre-oxidation and bonds with the external TiO QDs coating. The surface microstructures are analyzed by SEM, TEM and XPS methods, and the antimicrobial and anticoagulant performances are investigated by bacterial plate count and platelet adhesion tests. The oxidizing and hydrophilic effect of the top layer of TiO QDs were enhanced by the QD-sized particles. The antibacterial activities of the PDA/TiO QDs coating on PU catheters against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), especially to S. aureus, are evidenced by bacterial plate count test, reaching good bactericidal rates of 49.9 % against E. coli and 83.7 % against S. aureus, respectively. Platelet adhesion test and whole blood dynamic circulation modeling demonstrate that the PDA/TiO QDs coating effectively inhibits platelet adhesion due to an excellent hydrophilicity of TiO QDs surface, and thereafter reduce thrombus formation.
导管涂层可有效减少血流感染和血栓形成，而这两种情况是血液接触导管的主要并发症。然而，由于小口径导管产生的高表面拉伸力，表面功能涂层难以实现。在这项工作中，我们提出在聚氨酯（PU）导管上进行聚多巴胺/二氧化钛量子点（PDA/TiO QDs）的共价键涂层处理，这可以实现抗菌和抗血栓形成的双重功能。PDA/TiO QDs层通过浸涂制备，其中PDA的中间过渡层通过预氧化与PU表面的内部羟基反应，并与外部的TiO QDs涂层结合。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）和X射线光电子能谱（XPS）方法分析表面微观结构，并通过细菌平板计数和血小板黏附试验研究抗菌和抗凝性能。量子点尺寸的颗粒增强了TiO QDs顶层的氧化和亲水作用。细菌平板计数试验证明了PU导管上的PDA/TiO QDs涂层对大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus），尤其是对金黄色葡萄球菌的抗菌活性，对大肠杆菌和金黄色葡萄球菌的杀菌率分别达到49.9%和83.7%。血小板黏附试验和全血动态循环模型表明，由于TiO QDs表面具有优异的亲水性，PDA/TiO QDs涂层有效抑制血小板黏附，进而减少血栓形成。
