Holden B A, Williams L, Zantos S G
Invest Ophthalmol Vis Sci. 1985 Oct;26(10):1354-9.
To investigate the etiology of contact lens-induced transient endothelial changes (blebs) in the human cornea, the effects of five different stimuli on corneal thickness and the appearance of the corneal endothelium were assessed. The stimuli included: (1) a silicone contact lens; (2) a silicone contact lens in combination with anoxia; (3) anoxia alone; (4) a thick hydroxyethyl methacrylate (HEMA) contact lens; and (5) a gas mixture of 9.8% carbon dioxide, 20.5% oxygen, and the balance nitrogen. The silicone lens alone produced no significant alteration in endothelial appearance and little change in corneal thickness. However, when nitrogen gas was passed in front of the lens, a typical bleb response was observed. This indicates that the physical presence of a contact lens is insufficient by itself to produce transient endothelial changes. Anoxia alone induced corneal swelling and endothelial bleb formation, indicating a metabolic component in the bleb response. The gas mixture containing 9.8% carbon dioxide also altered the endothelial appearance but had no significant effect on corneal thickness. The thick HEMA lens produced changes in both the appearance of the endothelium and corneal thickness. The only factor common to the stimuli which induced blebs would appear to be their ability to change the pH in or near the corneal endothelial layer.
为了研究隐形眼镜引起的人眼角膜短暂内皮变化（小泡）的病因，评估了五种不同刺激对角膜厚度和角膜内皮外观的影响。刺激因素包括：（1）硅水凝胶隐形眼镜；（2）硅水凝胶隐形眼镜与缺氧相结合；（3）单独缺氧；（4）厚甲基丙烯酸羟乙酯（HEMA）隐形眼镜；（5）由9.8%二氧化碳、20.5%氧气和其余为氮气组成的混合气体。单独使用硅水凝胶镜片时，内皮外观无明显变化，角膜厚度变化也很小。然而，当氮气在镜片前通过时，观察到典型的小泡反应。这表明隐形眼镜本身的物理存在不足以产生短暂的内皮变化。单独缺氧会导致角膜肿胀和内皮小泡形成，表明小泡反应中存在代谢成分。含有9.8%二氧化碳的混合气体也会改变内皮外观，但对角膜厚度无显著影响。厚HEMA镜片会使内皮外观和角膜厚度都发生变化。诱导小泡形成的刺激因素中唯一共同的因素似乎是它们改变角膜内皮层或其附近pH值的能力。
