Department of Hand Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, 430070, China.
J Nanobiotechnology. 2022 Mar 19;20(1):147. doi: 10.1186/s12951-022-01354-4.
Clinical work and research on diabetic wound repair remain challenging globally. Although various conventional wound dressings have been continuously developed, the efficacy is unsatisfactory. The effect of drug delivery is limited by the depth of penetration. The sustained release of biomolecules from biological wound dressings is a promising treatment approach to wound healing. An assortment of cell-derived exosomes (exos) have been proved to be instrumental in tissue regeneration, and researchers are dedicated to developing biomolecules carriers with unique properties. Herein, we reported a methacrylate gelatin (GelMA) microneedles (MNs) patch to achieve transdermal and controlled release of exos and tazarotene. Our MNs patch comprising GelMA/PEGDA hydrogel has distinctive biological features that maintain the biological activity of exos and drugs in vitro. Additionally, its unique physical structure prevents it from being tightly attached to the skin of the wound, it promotes cell migration, angiogenesis by slowly releasing exos and tazarotene in the deep layer of the skin. The full-thickness cutaneous wound on a diabetic mouse model was carried out to demonstrate the therapeutic effects of GelMA/PEGDA@T + exos MNs patch. As a result, our GelMA/PEGDA@T + exos MNs patch presents a potentially valuable method for repairing diabetic wound in clinical applications.
临床工作和糖尿病创面修复研究仍然具有挑战性。尽管各种传统的创面敷料不断发展，但疗效并不理想。药物输送的效果受到穿透深度的限制。生物创面敷料中生物分子的持续释放是一种很有前途的创面愈合治疗方法。各种细胞来源的外泌体（exos）已被证明在组织再生中具有重要作用，研究人员致力于开发具有独特性能的生物分子载体。在这里，我们报道了一种甲基丙烯酸明胶（GelMA）微针（MNs）贴片，以实现外泌体和他扎罗汀的经皮和控制释放。我们的 MNs 贴片由 GelMA/PEGDA 水凝胶组成，具有独特的生物学特性，可在体外保持外泌体和药物的生物活性。此外，其独特的物理结构防止其紧密贴合伤口皮肤，通过在外泌体和他扎罗汀在皮肤深层缓慢释放，促进细胞迁移和血管生成。在糖尿病小鼠模型的全层皮肤伤口上进行了实验，以证明 GelMA/PEGDA@T + exos MNs 贴片的治疗效果。结果表明，我们的 GelMA/PEGDA@T + exos MNs 贴片为临床应用中修复糖尿病创面提供了一种有价值的方法。
