Hunan Engineering Technology Research Center for Bioactive Substance Discovery of Chinese Medicine, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China.
Changsha Concord Herbs Cultivation Technology Co., Ltd., Changsha, 410221, China.
Curr Microbiol. 2024 Sep 21;81(11):368. doi: 10.1007/s00284-024-03887-3.
In the present study, using genome mining, Streptomyces sp. JL1001, which possesses a leinamycin-type gene cluster, was identified from 14 strains of Streptomyces originating from the rhizosphere soil of Polygonatum cyrtonema Hua. The complete genome of Streptomyces sp. JL1001 was sequenced and analyzed. The genome of Streptomyces sp. JL1001 consists of a 7,943,495 bp chromosome with a 71.71% G+C content and 7315 protein-coding genes. We also identified 36 biosynthetic gene clusters (BGCs) for secondary metabolites in Streptomyces sp. JL1001. Twenty-seven BGCs had low (< 50%) or moderate (50-80%) similarity to other known secondary metabolite BGCs. In addition, a comparative analysis was conducted between the leinamycin-type gene cluster in Streptomyces sp. JL1001 and the biosynthetic gene clusters of leinamycin and largimycin. This study aims to provide a comprehensive analysis of the genomic features of rhizosphere Streptomyces sp. JL1001. It establishes the foundation for further investigation into experimental trials involving novel bioactive metabolites such as AT-less type I polyketides that have important potential applications in medicine and agriculture.
在本研究中，通过基因组挖掘，从来自多花黄精根际土壤的 14 株链霉菌中鉴定出了一株具有雷纳霉素型基因簇的链霉菌 sp. JL1001。对链霉菌 sp. JL1001 的全基因组进行了测序和分析。链霉菌 sp. JL1001 的基因组由一个 7,943,495 bp 的染色体组成，GC 含量为 71.71%，包含 7315 个蛋白编码基因。我们还鉴定出了 36 个次级代谢产物生物合成基因簇（BGCs）。其中 27 个 BGC 与其他已知次级代谢产物 BGC 的相似性较低（<50%）或中等（50-80%）。此外，还对链霉菌 sp. JL1001 中的雷纳霉素型基因簇与雷纳霉素和 largimycin 的生物合成基因簇进行了比较分析。本研究旨在对根际链霉菌 sp. JL1001 的基因组特征进行全面分析，为进一步研究具有重要医学和农业应用潜力的新型生物活性代谢物（如 AT 缺失型 I 型聚酮化合物）的实验试验奠定基础。
