Department of Chemistry, Universitas Tanjungpura, Pontianak, 78124, Indonesia.
Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR, 97331, USA.
Arch Microbiol. 2023 May 20;205(6):244. doi: 10.1007/s00203-023-03556-2.
Streptomyces sp. RS2 was isolated from an unidentified sponge collected around Randayan Island, Indonesia. The genome of Streptomyces sp. RS2 consists of a linear chromosome of 9,391,717 base pairs with 71.9% of G + C content, 8270 protein-coding genes, as well as 18 rRNA and 85 tRNA loci. Twenty-eight putative secondary metabolites biosynthetic gene clusters (BGCs) were identified in the genome sequence. Nine of them have 100% similarity to BGCs for albaflavenone, α-lipomycin, coelibactin, coelichelin, ectoine, geosmin, germicidin, hopene, and lanthionine (SapB). The remaining 19 BGCs have low (< 50%) or moderate (50-80%) similarity to other known secondary metabolite BGCs. Biological activity assays of extracts from 21 different cultures of the RS2 strain showed that SCB ASW was the best medium for the production of antimicrobial and cytotoxic compounds. Streptomyces sp. RS2 has great potential to be a producer of novel secondary metabolites, particularly those with antimicrobial and antitumor activities.
从印度尼西亚兰丹扬岛附近采集的一种未鉴定海绵中分离到一株链霉菌 RS2。链霉菌 RS2 的基因组由一条 9,391,717 碱基对的线性染色体组成，GC 含量为 71.9%，包含 8270 个蛋白编码基因以及 18 个 rRNA 和 85 个 tRNA 基因座。在基因组序列中鉴定出 28 个潜在的次生代谢产物生物合成基因簇（BGCs）。其中 9 个与 albaflavenone、α-lipomycin、coelibactin、coelichelin、ectoine、geosmin、germicidin、hopene 和 lanthionine（SapB）的 BGC 具有 100%的相似性。其余 19 个 BGC 与其他已知次级代谢物 BGC 的相似性较低（<50%）或中等（50-80%）。对 RS2 菌株的 21 种不同培养物提取物的生物活性测定表明，SCB ASW 是产生抗菌和细胞毒性化合物的最佳培养基。链霉菌 RS2 具有成为新型次生代谢产物的生产者的巨大潜力，特别是那些具有抗菌和抗肿瘤活性的产物。
