Lcn2CreERT2小鼠神经炎症和反应性星形胶质细胞纵向研究方案

Protocol for the longitudinal study of neuroinflammation and reactive astrocytes in Lcn2CreERT2 mice.

作者信息

Ubina Teresa, Agnew-Svoboda William, Figueroa Zoe A, Wilson Emma H, Fiacco Todd A, Riccomagno Martin M

机构信息

Neuroscience Graduate Program, Univeristy of California, Riverside, Riverside, CA 92521, USA; Department of Molecular, Cell, and Systems Biology, University of California, Riverside, Riverside, CA 92521, USA.

Neuroscience Graduate Program, Univeristy of California, Riverside, Riverside, CA 92521, USA; Division of Biomedical Sciences, University of California, Riverside, Riverside, CA 92521, USA.

出版信息

STAR Protoc. 2024 Dec 20;5(4):103322. doi: 10.1016/j.xpro.2024.103322. Epub 2024 Sep 19.

DOI:10.1016/j.xpro.2024.103322

PMID:39305485

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11437937/

Abstract

During brain disease, astrocytes can reprogram into a reactive state that alters many of their functions. Here, we present a protocol for studying neuroinflammation and reactive astrogliosis in mice using lipopolysaccharide (LPS) from E. coli. We describe steps for employing the Lcn2CreERT2 mouse crossed into a fluorescent Cre reporter line to label a subset of reactive astrocytes during and after inflammation. We then detail procedures for the longitudinal study of reactive astrocytes during the induction, progression, and/or resolution of astrogliosis. For complete details on the use and execution of this protocol, please refer to Agnew-Svoboda et al..

摘要

在脑部疾病期间，星形胶质细胞可重编程为反应性状态，从而改变其许多功能。在此，我们展示了一种使用来自大肠杆菌的脂多糖（LPS）研究小鼠神经炎症和反应性星形胶质细胞增生的方案。我们描述了将Lcn2CreERT2小鼠与荧光Cre报告基因系杂交，以在炎症期间和之后标记反应性星形胶质细胞亚群的步骤。然后，我们详细介绍了在星形胶质细胞增生的诱导、进展和/或消退过程中对反应性星形胶质细胞进行纵向研究的程序。有关本方案使用和执行的完整详细信息，请参阅Agnew-Svoboda等人的研究。