Fish Research Centre, Faculty of Agricultural Environmental Sciences, Arish University, El-Arish, Egypt.
Department of Animal Wealth Development, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
BMC Vet Res. 2024 Sep 21;20(1):427. doi: 10.1186/s12917-024-04257-8.
Curcumin, the main polyphenol component of turmeric powder, has garnered increasing attention as an effective supplement in fish diets. A comparative trial was conducted to evaluate the impacts of dietary supplementation with different forms of curcumin (free, in combination, or nanoparticles) on hemato-biochemical parameters, reproductive capacity, and related gene expressions of red tilapia (Oreochromis niloticus x O. mossambicus) broodstock. Fish (n = 168) were fed an isonitrogenous (30% CP), isocaloric (18.72 MJ kg - 1) diet containing basal diet (Control), 60 mg kg-1 of either free curcumin (Cur), curcumin/nano-curcumin blend (Cur/NCur), or nano-curcumin (NCur) for 56 days. Red tilapia broodstock (155 ± 5.65 g) were stocked at a male: female ratio of 1:3. Blood samples and gonads were collected to assess hemato-biochemical parameters, reproductive capacity, and related gene expression at the end of the feeding trial. The results indicated that the values of hematological parameters (RBCs, WBCs, hemoglobin), total protein, albumin values, and reproductive hormones (T, LH, and FSH) were significantly increased, while liver function enzymes were decreased in the NCur group (P < 0.05). Reproductive performances (GSI, gonad maturation, total number of fry per female) were significantly improved in the NCur group compared with those in other groups (p < 0.05). The expression of reproductive genes (CYP19A1A, FSHR, LHR, FOXL2A, ESR1, ESR2A, and PGR) were significantly up-regulated in the gonads of fish fed NCur. Collectively, feeding red tilapia diets containing NCur led to noticeably better results followed by Cur/NCur blend, then free Cur compared to the control diet. These results indicate the superiority of NCur over its free or blended form, suggesting that a diet containing about 60 mg/kg of NCur is beneficial for enhancing hemato-biochemical parameters, improving reproductive performance, and enhancing the gonadal architecture of red tilapia.
姜黄素是姜黄粉中的主要多酚类成分，作为鱼类饲料中的有效补充剂越来越受到关注。本试验比较了不同形式的姜黄素（游离型、复合型或纳米型）对奥利亚罗非鱼（奥利亚罗非鱼×莫桑比克罗非鱼）亲鱼血液生化参数、繁殖能力和相关基因表达的影响。将 168 尾（155±5.65g）鱼随机分为 4 组，每组 42 尾，雌雄比为 1:3，分别投喂基础饲料（对照组）、基础饲料+60mg/kg 游离姜黄素（Cur）、基础饲料+姜黄素/纳米姜黄素复合物（Cur/NCur）和基础饲料+纳米姜黄素（NCur），试验期 56d。试验结束时，采集血液和性腺，检测血液生化参数、繁殖性能和相关基因表达。结果表明，NCur 组红细胞（RBCs）、白细胞（WBCs）、血红蛋白（Hb）、总蛋白（TP）、白蛋白（ALB）和生殖激素（T、LH、FSH）值显著升高，肝功能酶（AST、ALT、ALP）显著降低（P<0.05）。与其他组相比，NCur 组的繁殖性能（GSI、性腺成熟度、每尾雌鱼产仔数）显著提高（P<0.05）。NCur 组鱼类性腺中生殖基因（CYP19A1A、FSHR、LHR、FOXL2A、ESR1、ESR2A 和 PGR）的表达显著上调。综上所述，与对照组相比，在饲料中添加 NCur 的奥利亚罗非鱼的血液生化参数、繁殖性能和性腺结构明显改善，效果优于添加 Cur/NCur 混合物或游离 Cur。这些结果表明，NCur 比游离或复合形式更具优势，饲料中添加约 60mg/kg 的 NCur 有利于提高奥利亚罗非鱼的血液生化参数、繁殖性能和增强性腺结构。
