School of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, Yantai 264005, China.
School of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, Yantai 264005, China.
J Hazard Mater. 2024 Dec 5;480:135927. doi: 10.1016/j.jhazmat.2024.135927. Epub 2024 Sep 21.
Phycocyanin (PC) is of great significance to biomedicine and water environmental safety. Hence, it is indispensable to develop facile and rapid method for PC determination. In this investigation, a system containing lateral flow chromatography (LFC) strip (which was deposited with molecularly imprinted polymer (MIP) capped CdTe quantum dots (QDs) based mesoporous structured coated silica nanoparticles, SiO@QDs@ms-MIP NPs) and miniaturized fluorimeter was first fabricated. In detail, a two-step strategy was utilized for preparation of SiO@QDs@ms-MIP NPs, which consisted of modification of CdTe QDs onto the silica NPs first, and synthesis of mesoporous imprinting shell by using PC as template molecule and cetyltrimethylammonium bromide (CTAB) as surfactant. After that, novel fluorescence NPs possessing specific recognition and sensitivity toward PC in seawater and lake water were acquired. The resulting fluorescent sensing system exhibited outstanding performances, which included excellent sensitivity (4.5 nmol/L), satisfactory specificity (imprinting factor, 2.31), appropriate linearity range (0.01-5 μmol/L), good recovery (96.0-101.7 %), excellent stability (relative standard deviation, RSD<1.1 %), wonderful reproducibility (RSD<1.1 %), and excellent anti-interference ability. The results of the fluorescent sensing system were superior to those of the commonly used ultraviolet (UV) method. The proposed strategy showed great potential for fast (<10 min) and convenient fluorescence detection of PC in real samples.
藻蓝蛋白（PC）对生物医药和水环境保护具有重要意义。因此，开发简便、快速的 PC 测定方法是必不可少的。本研究首次制备了含有侧向流动色谱（LFC）条（其上沉积了基于分子印迹聚合物（MIP）包被的 CdTe 量子点（QDs）的介孔结构涂覆的硅纳米粒子，SiO@QDs@ms-MIP NPs）和微型荧光计的系统。具体而言，采用两步策略制备了 SiO@QDs@ms-MIP NPs，该策略包括首先将 CdTe QDs 修饰到硅纳米粒子上，然后使用 PC 作为模板分子和十六烷基三甲基溴化铵（CTAB）作为表面活性剂合成介孔印迹壳。之后，获得了对海水和湖水具有特定识别和敏感性的新型荧光 NPs。所得荧光传感系统表现出出色的性能，包括优异的灵敏度（4.5 nmol/L）、令人满意的特异性（印迹因子，2.31）、适当的线性范围（0.01-5 μmol/L）、良好的回收率（96.0-101.7 %）、出色的稳定性（相对标准偏差，RSD<1.1 %）、出色的重现性（RSD<1.1 %）和出色的抗干扰能力。荧光传感系统的结果优于常用的紫外（UV）方法。该策略显示出在实际样品中快速（<10 分钟）和方便的荧光检测 PC 的巨大潜力。
