Mass Spectrometry Technology Access Center at the McDonnell Genome Institute, Washington University, St. Louis, MO, United States.
Genome Technology Access Center, McDonnell Genome Institute, Washington University, St. Louis, MO, United States.
Am J Clin Nutr. 2024 Nov;120(5):1114-1124. doi: 10.1016/j.ajcnut.2024.09.018. Epub 2024 Sep 21.
Bovine milk is a beneficial ingredient in teh treatment of malnutrition.
Our objectives were to determine the effect of dietary milk protein and milk carbohydrate on the intestinal permeability, fecal 16S rRNA gene configuration, and fecal metabolomics of children with moderate malnutrition.
This was a randomized, double-blind, controlled trial among 413 children with wasting in rural Sierra Leone who received 1 of the following 4 supplementary foods, which differed in sources of protein and carbohydrate: milk protein and milk carbohydrate (MPMC), milk protein and vegetable carbohydrate (MPVC), vegetable protein and milk carbohydrate (VPMC), or a control group consuming entirely vegetable-based food (VPVC). After 4 wk, urine and stool were collected from participants enrolled with mid-upper arm circumference of <12.1 cm. Urine was analyzed for lactulose excretion (%L). Stool samples were subjected to both 16S rRNA gene analysis to assess β-diversity and untargeted metabolomic abundance.
Among the 386 children who completed permeability testing, the mean difference (95% CI) in %L excretion as compared with VPVC was 0.01 (-0.05, 0.07) for MPMC, 0.05 (-0.01, 0.11) for MPVC, and 0.01 (-0.05, 0.07) for VPMC. Of the 374 children who provided a stool sample that was analyzed, the β-diversity among bacterial taxa was similar between dietary groups (P > 0.05 for all comparisons). No significant differences between dietary groups were seen among the 20 most abundant bacterial taxa. Among the 5769 unique metabolomic features identified, greater flavonoid levels in VPVC were seen.
Abnormal intestinal permeability do not improve with 4 wk of supplementary feeding. Fecal rRNA do not differ with consumption of different diets. This trial was registered at clinicaltrials.gov as NCT04216043.
牛奶是治疗营养不良的有益成分。
我们的目的是确定饮食中的牛奶蛋白和牛奶碳水化合物对患有中度营养不良的儿童的肠道通透性、粪便 16S rRNA 基因结构和粪便代谢组学的影响。
这是一项在塞拉利昂农村地区患有消瘦的 413 名儿童中进行的随机、双盲、对照试验，他们接受了以下 4 种补充食品中的 1 种，这些食品在蛋白质和碳水化合物的来源上有所不同：牛奶蛋白和牛奶碳水化合物（MPMC）、牛奶蛋白和植物性碳水化合物（MPVC）、植物性蛋白和牛奶碳水化合物（VPMC）或完全食用植物性食物的对照组（VPVC）。4 周后，从中臂围<12.1cm 的入组参与者中收集尿液和粪便。尿液用于分析乳果糖排泄量（%L）。粪便样本同时进行 16S rRNA 基因分析，以评估β多样性和非靶向代谢组学丰度。
在完成通透性测试的 386 名儿童中，与 VPVC 相比，MPMC、MPVC 和 VPMC 的%L 排泄量的平均差异（95%CI）分别为 0.01（-0.05，0.07）、0.05（-0.01，0.11）和 0.01（-0.05，0.07）。在提供可分析粪便样本的 374 名儿童中，不同饮食组之间细菌分类群的β多样性相似（所有比较的 P>0.05）。在 20 种最丰富的细菌分类群中，不同饮食组之间没有显著差异。在鉴定的 5769 个独特代谢组学特征中，VPVC 中黄酮类水平较高。
4 周的补充喂养并不能改善肠道通透性异常。粪便 rRNA 与不同饮食的摄入没有差异。本试验在 clinicaltrials.gov 上注册为 NCT04216043。
