Ibrahim Habibu Ahmad, Suardi Nursakinah, Khaniabadi Pegah Moradi, Zulbaharin Siti Farrah Mursyida, Taggo Aijesta
School of Physics, Universiti Sains Malaysia, 11800, Penang, Malaysia.
Department of Physics, Kano University of Science and Technology Wudil, Kano, Nigeria.
Lasers Med Sci. 2024 Sep 23;39(1):238. doi: 10.1007/s10103-024-04187-9.
Breast cancer remains a significant global health challenge, spurring ongoing investigations into innovative treatment approaches. Low-level laser therapy (LLLT) has emerged as a promising non-invasive therapeutic avenue of interest. This research delves into the impact of LLLT on the cytotoxicity of the MCF-7 breast cancer cell line, employing lasers emitting various wavelengths. The objective is to assess whether diverse LLLT wavelengths elicit disparate cytotoxic responses, shedding light on LLLT's potential as a targeted breast cancer treatment. MCF-7 cell cultures were subjected to lasers of varying wavelengths, including blue (473 nm), red (660 nm), and near-infrared (780 nm). Each wavelength was delivered at four different power levels: 10, 25, 45, and 65 mW, with exposure durations of 60, 300, 600, and 900 s. Cellular responses, encompassing factors such as cell viability, and cytotoxicity were assessed using WST-1 assays technique. Statistical analysis was performed to discern the wavelength-specific impacts of low-level laser therapy (LLLT) on MCF-7 cells. The study revealed that the blue laser had the least noticeable adverse impact on MCF-7 breast cancer cell lines, leading to the highest cell survival rate of 107.62% after 24 h. The most severe toxicity occurred when the laser was used at 45 mW for 900 s, resulting in cell viability ranging from 81.85% to 107.62%. As for cell viability after exposure to the red laser, the mildest harmful effect was observed at 45 mW power for 60 s, resulting in a cell survival rate of 147.62%. Conversely, the most significant toxic response occurred at 10 mW power for 60 s, resulting in a cell viability of 91.56%. In contrast, when employing infrared laser irradiation, the least substantial cytotoxic effect on MCF-7 cells was observed at 10 mW power for 600 s, resulting in the highest cell viability of 109.37% after 24 h. The most pronounced cytotoxic effect was observed by infrared laser (780 nm) at 25 mW power for 900 s, leading to the lowest viability of 32.53%.
乳腺癌仍然是一项重大的全球健康挑战，这促使人们不断对创新治疗方法展开研究。低强度激光疗法（LLLT）已成为一种颇具前景的无创治疗途径。本研究深入探讨了LLLT对MCF-7乳腺癌细胞系细胞毒性的影响，采用了发射不同波长的激光。目的是评估不同的LLLT波长是否会引发不同的细胞毒性反应，从而揭示LLLT作为一种靶向乳腺癌治疗方法的潜力。MCF-7细胞培养物接受了不同波长的激光照射，包括蓝色（473纳米）、红色（660纳米）和近红外（780纳米）。每个波长以四种不同的功率水平发射：10、25、45和65毫瓦，照射持续时间分别为60、300、600和900秒。使用WST-1检测技术评估细胞反应，包括细胞活力等因素以及细胞毒性。进行统计分析以辨别低强度激光疗法（LLLT）对MCF-7细胞的波长特异性影响。研究表明，蓝色激光对MCF-7乳腺癌细胞系的不良影响最小，24小时后细胞存活率最高，达到107.62%。当激光以45毫瓦照射900秒时，毒性最为严重，细胞活力在81.85%至107.62%之间。至于暴露于红色激光后的细胞活力，在45毫瓦功率照射60秒时观察到的有害影响最轻，细胞存活率为147.62%。相反，在10毫瓦功率照射60秒时出现了最显著的毒性反应，细胞活力为91.56%。相比之下，当采用红外激光照射时，在10毫瓦功率照射600秒时观察到对MCF-7细胞的细胞毒性作用最小，24小时后细胞活力最高，达到109.37%。红外激光（780纳米）在25毫瓦功率照射900秒时观察到最明显的细胞毒性作用，导致最低的细胞活力为32.53%。
