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奶酪样品中与相关肠毒素基因的分子鉴定。

Molecular identification of -related enterotoxin genes in cheese samples.

机构信息

Collage of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah City, Iraq.

Collage of Biotechnology, University of Al-Qadisiyah, Al-Diwaniyah City, Iraq.

出版信息

Open Vet J. 2024 Aug;14(8):2073-2078. doi: 10.5455/OVJ.2024.v14.i8.36. Epub 2024 Aug 31.

Abstract

BACKGROUND

Dairy products are considered some important sources of various nutritional compounds; however, pathogenic bacterial growth is a critical destructive factor to these products leading to consumer health and system financial crises.

AIM

The current study was carried out to identify if there is any presence of -related enterotoxin genes in cheese samples.

METHODS

The research included the collection of 35 samples. The samples passed through conventional cultivation processes and a PCR method to detect the presence of , , , and enterotoxin genes in these samples.

RESULTS

The conventional identification revealed the growth of . from the cheese samples. The PCR findings recorded the presence of the , , , and in 31 (88.5%), 27 (77%), 19 (54%), and 12 (34%), respectively, of cheese samples. The sequencing revealed close similarities with global isolates, which reached up to 98.5% of identity.

CONCLUSION

The current results indicate the presence of enterotoxin genes of in high rates in the dairy products examined, which reveals critical problems of food safety due to the possible presence of enterotoxins in consumer dairy products.

摘要

背景

乳制品被认为是各种营养化合物的重要来源之一;然而,病原菌的生长是对这些产品的一个关键破坏性因素,会导致消费者健康和系统金融危机。

目的

本研究旨在确定奶酪样品中是否存在与相关的肠毒素基因。

方法

该研究包括收集 35 个样本。这些样本经过常规培养过程和 PCR 方法检测,以检测这些样本中 是否存在 、 、 、 和 肠毒素基因。

结果

常规鉴定显示奶酪样品中存在 。PCR 结果记录了 31 个(88.5%)、27 个(77%)、19 个(54%)和 12 个(34%)奶酪样品中 、 、 、 和 的存在。测序结果与全球分离株具有高度相似性,达到 98.5%的同一性。

结论

目前的结果表明,在所检查的乳制品中存在高比率的 肠毒素基因,这表明由于消费者乳制品中可能存在肠毒素,存在食品安全的重大问题。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91ff/11415922/1142c253518b/OpenVetJ-14-2073-g001.jpg

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