Meng Fan, Liang Chaohao, Ali Barkat, Wan Changwu, He Fengbing, Chen Jiarui, Zhang Yiqing, Luo Zhijia, Su Lingling, Zhao Xiaoya, Yang Bin, Zhang Jian
The Key Laboratory of Advanced Interdisciplinary Studies, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, Guangdong 510120, PR China.
School of Biomedical Engineering, Guangzhou Medical University, Guangzhou, Guangdong 510182, PR China.
Photoacoustics. 2024 Sep 7;39:100643. doi: 10.1016/j.pacs.2024.100643. eCollection 2024 Oct.
Vaccine development requires high-resolution, in situ, and visual adjuvant technology. To address this need, this work proposed a novel adjuvant labeling that involved indocyanine green (ICG) and bovine serum albumin (BSA) with self-assembled aluminium adjuvant (Alum), which was called BSA@ICG@Alum. This compound exhibited excellent photoacoustic properties and has been confirmed its safety, biocompatibility, high antigen binding efficiency, and superior induction of immune response. Photoacoustic tomography (PAT) tracked the distribution of Alum in lymph nodes (LNs) and lymphatic vessels in real time after diverse injection modalities. The non-invasive imaging approach revealed that BSA@ICG@Alum was transported to the draining LNs 60 min after intramuscular injection and to distal LNs within 30 min after lymph node injection. In conclusion, PAT enabled real-time three-dimensional and quantitative visualization, thus offering a powerful tool for advancing vaccine design by providing critical insights into adjuvant transport and immune system activation.
疫苗研发需要高分辨率、原位和可视化的佐剂技术。为满足这一需求，本研究提出了一种新型佐剂标记物，它由吲哚菁绿（ICG）、牛血清白蛋白（BSA）和自组装铝佐剂（明矾）组成，称为BSA@ICG@明矾。该化合物具有优异的光声特性，并已证实其安全性、生物相容性、高抗原结合效率以及卓越的免疫反应诱导能力。光声断层扫描（PAT）在采用不同注射方式后实时追踪了明矾在淋巴结（LN）和淋巴管中的分布。这种非侵入性成像方法显示，肌肉注射后60分钟，BSA@ICG@明矾被转运至引流淋巴结，淋巴结注射后30分钟内被转运至远端淋巴结。总之，PAT实现了实时三维定量可视化，从而通过提供关于佐剂转运和免疫系统激活的关键见解，为推进疫苗设计提供了一个强大的工具。
