Gao Hongjuan, Qin Han, Fu Hongjing, Feng Jing, Chen Min
Operating Room, Wuhan Fourth Hospital, Wuhan, 430000, China.
Department of Anesthesiology, Wuhan Fourth Hospital, Wuhan, 430000, China.
Heliyon. 2024 Sep 5;10(18):e37449. doi: 10.1016/j.heliyon.2024.e37449. eCollection 2024 Sep 30.
General anesthesia is significantly gaining prominence and becoming unavoidable in modern medicine. Since neuroprotein fluctuations are common during anesthetic procedures, it is essential to monitor protein levels to identify neuro-related issues. Tau protein fluctuations are often found in the anesthetic process, and higher levels of tau are highly related to various neuro-related issues. Researchers are focusing on monitoring tau levels during and after anesthesia. This research has developed a high-sensitive tau biosensor on a gold nanomaterial-modified interdigitated electrode, measured at 0-2 V on a dual-probe station. Aptamer and antibody were used as capture and detection molecules, and a biotin-streptavidin strategy was employed to attach a higher number of aptamers on the electrode. These immobilized aptamers recognize the tau protein and form a sandwich with antibodies, lowering the detection of tau protein to 1 fM on a linear regression from 0.001 to 100 pM (y = 2.0651x - 1.3813, R = 0.987). Further, tau-spiked cerebrospinal fluid increases the current flow without any interferences, confirming the selective detection of tau protein.
全身麻醉在现代医学中日益凸显其重要性且变得不可或缺。由于在麻醉过程中神经蛋白波动很常见，监测蛋白质水平以识别神经相关问题至关重要。在麻醉过程中经常发现tau蛋白波动，较高水平的tau与各种神经相关问题高度相关。研究人员正专注于在麻醉期间及之后监测tau水平。这项研究在金纳米材料修饰的叉指电极上开发了一种高灵敏度的tau生物传感器，在双探针站上于0至2伏进行测量。适体和抗体被用作捕获和检测分子，并采用生物素-链霉亲和素策略在电极上附着更多数量的适体。这些固定化的适体识别tau蛋白并与抗体形成夹心结构，在0.001至100皮摩尔的线性回归中，将tau蛋白的检测下限降低至1飞摩尔（y = 2.0651x - 1.3813，R = 0.987）。此外，添加了tau的脑脊液增加了电流流动且无任何干扰，证实了对tau蛋白的选择性检测。
