Yang Bin, Tian Faming, Yu Huilin
Department of Orthopaedic Centre, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, 758 Hefei Road, Qingdao, 266000, Shandong, China.
Qingdao Medical Engineering Interdisciplinary Key Laboratory, Qilu Hospital (Qingdao), Shandong University, 758 Hefei Road, Qingdao, 266000, Shandong, China.
Heliyon. 2024 Jul 22;10(16):e34677. doi: 10.1016/j.heliyon.2024.e34677. eCollection 2024 Aug 30.
Septic arthritis (SA) is more severe in patients with rheumatoid arthritis, joint surgical issues, or a weakened immune system. Timely diagnosis of SA is crucial for effective treatment. Traditional diagnostic methods such as ELISA, white blood cell counting, blood culture, qPCR, and imaging techniques are often less accurate and time-consuming. Researchers are focusing on developing highly sensitive biosensors for SA using blood-based biomarkers. Procalcitonin is a protein and a well-established biomarker for SA. This research focuses on developing a procalcitonin interdigitated electrode (IDE) biosensor using a probe made of an aptamer and antibody-modified gold nanoparticle (AuNP) complex. The probe was attached to the IDE through an amine linker and then interacted with procalcitonin. AuNPs increased the attachment of the aptamer and antibody to the IDE, enabling the detection of procalcitonin at levels as low as 10 ng/mL, with a linear regression curve ranging from 10 to 100 ng/mL [y = 4.0691x - 2.1887; R = 0.9937]. Furthermore, procalcitonin-spiked serum elevated the current level with increasing procalcitonin concentrations, while control performances did not enhance the current, indicating the selective and specific detection of procalcitonin. This AuNP-aptamer-antibody complexed biosensor effectively identifies procalcitonin at low levels and aids in the diagnosis of SA.
脓毒性关节炎（SA）在类风湿性关节炎、关节手术问题或免疫系统较弱的患者中更为严重。SA的及时诊断对于有效治疗至关重要。传统的诊断方法，如酶联免疫吸附测定（ELISA）、白细胞计数、血培养、定量聚合酶链反应（qPCR）和成像技术，往往准确性较低且耗时较长。研究人员正致力于开发基于血液生物标志物的用于SA的高灵敏度生物传感器。降钙素原是一种蛋白质，是SA一种公认的生物标志物。本研究聚焦于使用由适配体和抗体修饰的金纳米颗粒（AuNP）复合物制成的探针开发一种降钙素原叉指电极（IDE）生物传感器。该探针通过胺连接子连接到IDE上，然后与降钙素原相互作用。金纳米颗粒增加了适配体和抗体与IDE的附着，能够检测低至10纳克/毫升水平的降钙素原，线性回归曲线范围为10至100纳克/毫升[y = 4.0691x - 2.1887；R = 0.9937]。此外，添加了降钙素原的血清随着降钙素原浓度的增加提高了电流水平，而对照样本未增强电流，这表明该生物传感器对降钙素原具有选择性和特异性检测能力。这种金纳米颗粒 - 适配体 - 抗体复合生物传感器能有效识别低水平的降钙素原，有助于SA的诊断。
