Fu Qiafan, Yu Zengying, Yang Changquan, Yu Mengmeng, Wei Yanxue, Wang Chaoyue
Department of Pharmacy, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, 365 East Renmin Road, 321000 Jinhua, P. R. China.
Xinzhi College, Zhejiang Normal University, Lanxi, 321100 Jinhua, P. R. China.
ACS Omega. 2024 Sep 4;9(37):38490-38497. doi: 10.1021/acsomega.4c03065. eCollection 2024 Sep 17.
Jinhua finger citron is one of the traditional specialties of Jinhua with a long history of cultivation. The current study highlighted the advantages of using the quality by design approach to optimize the ultrasonic-assisted distillation extraction of Jinhua finger citron essential oil. The yield of Jinhua finger citron essential oil was regarded as a potential critical quality attribute. Potential critical process parameters were screened by the definitive screening design. Finally, the design space of the essential oil extraction process was constructed. The optimal operating space included an auxiliary NaCl concentration range of 9-12.00%, a soak temperature range of 30-50 °C, a distillation time range of 3.5-4.00 h, an ultrasonic power range of 200-300 W, a solid-liquid ratio range of 1:3-1:3.5, and a soak time range of 40-80 min. On this basis, the relative density, refractive index, and pH values of different batches of Jinhua finger citron essential oil were checked. The involved batches were analyzed by gas chromatography (GC), and gas chromatographic fingerprints were established by identifying major compounds, including d-limonene, γ-terpinene, and 5,7-dimethoxycoumarin. Based on the "quality by design" strategy, the extraction process of Jinhua finger citron essential oil established in this study was robust, reliable, and flexible.
金华佛手是金华的传统特产之一，种植历史悠久。当前的研究突出了采用质量源于设计方法来优化金华佛手精油的超声辅助蒸馏提取的优势。将金华佛手精油的产量视为一个潜在的关键质量属性。通过析因筛选设计来筛选潜在的关键工艺参数。最后，构建了精油提取工艺的设计空间。最佳操作空间包括辅助氯化钠浓度范围为9 - 12.00%、浸泡温度范围为30 - 50℃、蒸馏时间范围为3.5 - 4.00小时、超声功率范围为200 - 300瓦、固液比范围为1:3 - 1:3.5以及浸泡时间范围为40 - 80分钟。在此基础上，检测了不同批次金华佛手精油的相对密度、折射率和pH值。对所涉及的批次进行气相色谱（GC）分析，并通过鉴定主要化合物（包括d - 柠檬烯、γ - 松油烯和5,7 - 二甲氧基香豆素）建立了气相色谱指纹图谱。基于“质量源于设计”策略，本研究建立的金华佛手精油提取工艺稳健、可靠且灵活。
